SILAC

Methods and Protocols

Jose L. Luque-Garcia

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English

Springer-Verlag New York Inc.
14 November 2023

Scientific equipment, experiments & techniques; Proteins; Cellular biology (cytology)

Series: Methods in Molecular Biology

Summary
Details

This detailed volume explores the most recent methodologies and applications of stable isotope labeling by amino acids in cell culture (SILAC). With the development of new technologies in the field of mass spectrometry and bioinformatics, SILAC-based methods have become the first tool of choice in quantitative proteomics either on its own or in combination with other more recently developed approaches, which is elucidated in the chapters of this book. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, SILAC: Methods and Protocols serves as an ideal guide for researchers working in the area of functional proteomics and other aspects of protein science.

Edited by: Jose L. Luque-Garcia
Imprint: Springer-Verlag New York Inc.
Country of Publication: United States
Edition: 2023 ed.
Volume: 2603
Dimensions: Height: 254mm, Width: 178mm,
Weight: 574g
ISBN: 9781071628652
ISBN 10: 1071628658
Series: Methods in Molecular Biology
Pages: 286
Publication Date: 14 November 2023
Audience: Professional and scholarly , Undergraduate
Format: Paperback
Publisher's Status: Active