Multiprotein Complexes

Methods and Protocols

Arnaud Poterszman

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English

Springer-Verlag New York Inc.
12 December 2021

Proteins

Series: Methods in Molecular Biology

Summary
Details

This volume explores strategies and detailed protocols for the preparation of macromolecular complexes and their characterization in view of structural analysis. The chapters in this book are separated into three parts: Part One focuses on sample preparation, and covers strategies for recombinant expression of multiprotein complexes in prokaryotic and eukaryotic hosts, for genome engineering using the CRISPR/Cas9 system and for production of specific binders such as reformatted antibodies and artificial binding proteins. Part Two looks at the biophysical methods that can provide useful indicators for sample optimization, and often complement structural information obtained with core technologies for structure determination—x-ray crystallography and cryo-electron microscopy—by quantitative solution data. Part Three discusses the characterization of multiprotein complexes in a cellular environment using the latest technologies and in vivo approaches. Written in the highly successfulMethods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Multiprotein Complexes: Methods and Protocols is a valuable resource for structural and molecular biologists who need to prepare multi-components for their applications, and for other scientists working on macromolecular assemblies from other angles that need to know the latest approaches that the field has to offer.

Edited by: Arnaud Poterszman
Imprint: Springer-Verlag New York Inc.
Country of Publication: United States
Edition: 2021 ed.
Volume: 2247
Dimensions: Height: 254mm, Width: 178mm,
Weight: 669g
ISBN: 9781071611289
ISBN 10: 1071611283
Series: Methods in Molecular Biology
Pages: 340
Publication Date: 12 December 2021
Audience: Professional and scholarly , Undergraduate
Format: Paperback
Publisher's Status: Active