Nanoscopy and Multidimensional Optical Fluorescence Microscopy

Alberto Diaspro

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English

Chapman & Hall/CRC
26 April 2010

Light (optics)

"""Alberto Diaspro has been choreographing light's dance for over 20 years,

and in Nanoscopy and Multidimensional Optical Fluorescence Microscopy, he has assembled a diverse group of experts to explain the methods they use to coax light to reveal biology's secrets."" -- From the Foreword by Daniel Evanko, editor, Nature Methods

Nanoscopy and Multidimensional Optical Fluorescence Microscopy demonstrates that the boundaries between sciences do blur at the bottom, especially those that might separate the optical work of physicists and the cellular work of microbiologists. In 18 chapters written by pioneering researchers, this work offers the first comprehensive and current documentation of the cutting-edge research being accomplished in a wide range of photonic devices with revolutionary application.

The highlight of the book is its coverage of optical nanoscopy and super-resolution microscopy. The rapid advances in this area over the past few years offer researchers in both photonics and molecular biologya wealth of accomplishment upon which they can build. Offering a complete treatment of this emerging field, this volume: Describes how scientists have exploited the properties of light and its fluorophore partners to overcome the resolution limit of conventional light microscopy

Delves into recent ways to minimize the photobleaching that has long hampered many methods including those that have the potential to capture previously unobtainable information on the movements of single molecules

Discusses the principles, benefits, and implementation of fluorescence correlation spectroscopy and related methods, which simplifies analysis by limiting light to stationary focal points in a sample

Considers the most basic as well as emerging methods for improving three-dimensional optical sectioning microscopy

Reviews the basics of FRET (fluorescence resonance energy transfer) and considers its new use for investigating protein complexes

The text also introduces those emerging nonfluorescence microscopy methods that can actually exert mechanical forces to trap and move a variety of objects ranging from beads to living cells and cellular organelles. Combining this technique with fluorescence microscopy provides an unparalleled ability to manipulate and visualize biological samples.

In the half-century since Richard Feynman challenged scientists to come up with the tools to investigate and manipulate our world at the nanoscale, we have succeeded in placing tools in the hands of biophysicists that are leading to major breakthroughs in our understanding of life and our ability to diagnose, treat, and prevent many challenges to human health. This book reflects what has been accomplished to date while pointing the way to what still needs to be done."

Edited by: Alberto Diaspro
Imprint: Chapman & Hall/CRC
Country of Publication: United Kingdom
Dimensions: Height: 254mm, Width: 178mm, Spine: 25mm
Weight: 952g
ISBN: 9781420078862
ISBN 10: 1420078860
Pages: 448
Publication Date: 26 April 2010
Audience: Professional and scholarly , Undergraduate
Format: Hardback
Publisher's Status: Active

Optical Microsopy and Nanoscopy. Basis if Fluorescence Spectroscopy. Basis of Optics for Microscopy. Scanning Microscopy at the Nanoscale. 3D Computational Optical Sectioning Microscopy. Multiphoton Microscopy at the Nanoscale. Confocal Microscopy at the Nanoscale. 7D Microscopy. Lifetime Imaging Microscopy. Fluorescence Correlation Spectroscopy. FRET/FRAP. Single Molecule Microscopy. Single Particle Tracking (SPT). Fluorescent Probe. Fluorescence Photoactivation and Photoswitching. Fluorescent Nanotubes and Nanowires. Scattering Processes. SHG/THG (Second/Third Harmonic Generation) Microscopy. Near Field Microscopy. TIRF (Total Internal Reflection Fluorescence) Microscopy.

Alberto Diaspro

Reviews for Nanoscopy and Multidimensional Optical Fluorescence Microscopy

This book certainly appears at the right time ! . The book should be useful for senior research students in optical microscopy and biophotonics. --Professor Min Gu, Swinburne University of Technology, Melbourne, Australia A very useful book for the microscopy field. Alberto Diaspro has done a great job as editor, and I'm sure this book will be appreciated by its readers. --Professor Peter Saggau, Baylor College of Medicine, Texas, USA ! with over sixty contributing authors, this elegant book should impress many optical manufacturers ! --Optical World, August 2010