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Super-Resolution Microscopy

A Practical Guide

Udo J. Birk

$213.95

Paperback

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English
Blackwell Verlag GmbH
11 October 2017
This unique book on super-resolution microscopy techniques presents comparative, in-depth analyses of the strengths and weaknesses of the individual approaches. It was written for non-experts who need to understand the principles of super-resolution or who wish to use recently commercialized instruments as well as for professionals who plan to realize novel microscopic devices. Explaining the practical requirements in terms of hardware, software and sample preparation, the book offers a wealth of hands-on tips and practical tricks to get a setup running, provides invaluable help and support for successful data acquisition and specific advice in the context of data analysis and visualization. Furthermore, it addresses a wide array of transdisciplinary fields of applications.

The author begins by outlining the joint efforts that have led to achieving super-resolution microscopy combining advances in single-molecule photo-physics, fluorophore design and fluorescent labeling, instrument design and software development. The following chapters depict and compare current main standard techniques such as structured illumination microscopy, single-molecule localization, stimulated emission depletion microscopy and multi-scale imaging including light-sheet and expansion microscopy. For each individual approach the experimental setups are introduced, the imaging protocols are provided and the various applications illustrated. The book concludes with a discussion of future challenges addressing issues of routine applications and further commercialization of the available methods.

Guiding users in how to make choices for the design of their own experiments from scratch to promising application, this one-stop resource is intended for researchers in the applied sciences, from chemistry to biology and medicine to physics and engineering.

By:  
Imprint:   Blackwell Verlag GmbH
Country of Publication:   Germany
Dimensions:   Height: 244mm,  Width: 170mm,  Spine: 20mm
Weight:   953g
ISBN:   9783527341337
ISBN 10:   3527341331
Pages:   408
Publication Date:  
Audience:   Professional and scholarly ,  Undergraduate
Format:   Paperback
Publisher's Status:   Active

Udo Birk studied physics and mathematics in Canada and Germany and obtained a PhD in applied physics from the University of Heidelberg (Germany) for his work on tissue spectrometry and spatially modulated illumination microscopy. He was a Marie-Curie Fellow at King's College London (UK) and at the Foundation of Research and Technology Hellas (Greece), where he worked on structured illumination microscopy and on optical projection tomography. Currently, he is deputy group leader at the Institute of Molecular Biology in Mainz (Germany), which equals a position of an associate professor, and his research focuses on the development and application of advanced optical imaging techniques.

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