High-performance Liquid Chromatography And Mass Spectrometry Of Porphyrins, Chlorophylls And Bilins

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Chang-kee Lim (Birkbeck, Univ Of London, Uk)

High-performance Liquid Chromatography And Mass Spectrometry Of Porphyrins, Chlorophylls And Bilins

Chang-kee Lim (Birkbeck, Univ Of London, Uk)

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English

World Scientific Publishing Co Pte Ltd
01 January 2024

Clinical & internal medicine; Chromatography; Biochemistry

Series: Methods In Chromatography

Summary
Details

Presents a compilation of HPLC methods for the separation and quantitation of porphyrins and porphyrin precursors. The physical-chemical background, structure-retention relationships and optimization of separation of porphyrins and porphyrinogens by reversed-phase HPLC are discussed. There are practical tips based on application-oriented examples, including detailed instructions and protocols, from sample collection, handling and storage preparation, and HPLC separation and quantitation. Methods for measurement of haem biosynthetic enzyme activities are described, and characteristic porphyrin profiles in the blood, urine and faeces of patients with different types of porphyrias are given. There are also chapters relating to TLC and capillary electrophoresis (CE) of porphyrins.

By: Chang-kee Lim (Birkbeck Univ Of London Uk)
Imprint: World Scientific Publishing Co Pte Ltd
Country of Publication: Singapore
Volume: 2
Dimensions: Height: 231mm, Width: 152mm, Spine: 18mm
Weight: 476g
ISBN: 9789810230685
ISBN 10: 9810230680
Series: Methods In Chromatography
Pages: 244
Publication Date: 01 January 2024
Audience: Professional and scholarly , Undergraduate
Format: Hardback
Publisher's Status: Unspecified