Mass Spectrometry

Techniques for Structural Characterization of Glycans

Michael A. Madson (BioLogistics, LLC, Iowa, USA)

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English

Elsevier Science Publishing Co Inc
16 May 2016

Spectrum analysis, spectrochemistry, mass spectrometry; Enzymology

Mass Spectrometry: Techniques for the Structural Characterization of Glycans presents new methods for conducting detailed carbohydrate qualitative analysis—arming analytical chemists, pharmaceutical scientists, and food scientists with a quick reference that will allow them to determine the structures of carbohydrates molecules.

As there is a need in the scientific community for content specific to structural determination and analysis of new glycoprotein drug, and because structure-activity analysis requires a structural determination of the N- and O-linked oligosaccharides linked to glycol-proteins, this book provides the relevant research that are necessary for advances and new outcomes in this area of study.

By: Michael A. Madson (BioLogistics LLC Iowa USA)
Imprint: Elsevier Science Publishing Co Inc
Country of Publication: United States
Dimensions: Height: 229mm, Width: 151mm, Spine: 10mm
Weight: 320g
ISBN: 9780128041291
ISBN 10: 0128041293
Pages: 86
Publication Date: 16 May 2016
Audience: Professional and scholarly , Undergraduate
Format: Hardback
Publisher's Status: Active

• Introduction• Monosaccharide Composition Analysis• Elemental Analysis of MS Ions• Derivatization Techniques• Hydride Addition to Sulfate and phosphate- Chemistry• InstrumentationMALDI-TOF MSAPI 2000 MSICP ESI TOF MS• Hydride Insertion Addition Reaction for the Derivatization of Phosphorylated or Sulfated Glycans and the Discernment of Phosphate versus Sulfate Esters• Ester Substitution MS MethodThe Protocol for the Hydride Addition to the ethanol extract of banana fruitExample of MS2on Hydride added to the ethanol Extract of Banana Fruit Glycan• Glycan Isolation Methods• Other Glycan isolation Methods• Monosaccharide AnalysisAcid Hydrolysis• Mass Spectral Carbohydrate Structure Determination MethodMSMS/MS (MS2)MSnProposing Initial Structure Refining Methods for Determining Exact Mass StructureMS Instrumentation Requirements for Discovering Whole Molecule IonsDerivatization Method and Sulfate/Phosphate Discernment Method• References

Mike Madson is CEO and Senior Research Scientist of BioLogistics, LLC, Iowa, USA