Mass Spectrometry of Glycoproteins

Methods and Protocols

Arnaud Delobel

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English

Springer-Verlag New York Inc.
29 April 2021

Chemistry; Spectrum analysis, spectrochemistry, mass spectrometry

Series: Methods in Molecular Biology

Summary
Details

This volume presents methods used for the analysis of glycoproteins at different levels—intact, subunit, glycopeptide, and monosaccharide--, and discusses and solves most analytical challenges that a scientist working on glycoproteins may come across. The chapters in this book cover topics such as the role of glycosylation on the properties of therapeutic glycoproteins; different analytical methods to characterize glycosylation, from the intact proteins to the glycan level, for both N-linked and O-linked glycoproteins; mass spectrometry imaging methodology for glycosylation analysis in tissues; approaches to characterizing glycosylation on cultured cells; and the use of cloud computing to deploy mass spectrometry data analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Mass Spectrometry of Glycoproteins: Methods and Protocols is a valuable resource for scientists interested in learning more about this developing field.

Edited by: Arnaud Delobel
Imprint: Springer-Verlag New York Inc.
Country of Publication: United States
Edition: 1st ed. 2021
Volume: 2271
Dimensions: Height: 254mm, Width: 178mm,
Weight: 976g
ISBN: 9781071612408
ISBN 10: 1071612409
Series: Methods in Molecular Biology
Pages: 401
Publication Date: 29 April 2021
Audience: Professional and scholarly , Undergraduate
Format: Hardback
Publisher's Status: Active