In Situ Hybridization in Electron Microscopy

Gerard Morel, Annie Cavalier, Lynda Williams (The Rowett Research Institute, Aberdeen, UK)

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English

CRC Press
18 December 2020

Microscopy; Physics; Life sciences: general issues; Molecular biology; Cellular biology (cytology)

In situ hybridization is a technique that allows for the visualization of specific DNA and RNA sequences in individual cells, and is an especially important method for studying nucleic acids in heterogeneous cell populations. in situ Hybridization in Electron Microscopy reviews the three main methods developed for the ultrastructural visualization of genes:

° hybridization on ultrathin sections of tissue embedded in hydrophilic resin (post-embedding method) ° hybridization prior to embedding (pre-embedding) ° hybridization on ultrathin sections of frozen tissue (frozen tissue method).

For each technique, the different stages are described in detail: the preparation of tissue, pretreatment, hybridization, and visualization of the hybridization products.

The book combines theory and practice, starting with the basic principles, then breaking down the experimental process into successive steps illustrated by numerous diagrams, detailed protocols, and tables. This is all done in a format that uses parallel columns to convey useful comments next to the theory and practical details alongside each stage of the protocol. Additionally, the summary tables provide the criteria for choosing the probe type and technique, and a detailed index aids in the search for information. in situ Hybridization In Electron Microscopy is an essential companion for applying these methods at the electron microscopic level.

By: Gerard Morel, Annie Cavalier, Lynda Williams (The Rowett Research Institute, Aberdeen, UK)
Imprint: CRC Press
Country of Publication: United Kingdom
Dimensions: Height: 254mm, Width: 178mm,
Weight: 453g
ISBN: 9780367455378
ISBN 10: 0367455374
Pages: 472
Publication Date: 18 December 2020
Audience: Professional and scholarly , Undergraduate
Format: Paperback
Publisher's Status: Active

Morel, Gerard | Cavalier, Annie | Williams, Lynda