Histone Methyltransferases

Methods and Protocols

Raphaël Margueron, Daniel Holoch

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English

Springer-Verlag New York Inc.
24 June 2022

Genetics (non-medical); Enzymology

Series: Methods in Molecular Biology

Summary
Details

This volume provides methods used to investigate histone methyltransferase function. Chapters guide readers through a comprehensive set of approaches that detail phylogenetic diversity, histone demethylase activities in vitro, generating chromatin substrates, auto-methylation, quantification of metabolites, protein purification, crystallization, X-ray structure, cryogenic electron microscopy, assessing genome-wide patterns, CUT&Tag in mouse embryonic tissues, chemical biology approaches, peptide SPOT arrays, nascent chromatin capture, ectopic protein tethering, computational models, and development of methyltransferase inhibitors. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.

Authoritative and cutting-edge, Histone Methyltransferases: Methods and Protocols aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge.

Edited by: Raphaël Margueron, Daniel Holoch
Imprint: Springer-Verlag New York Inc.
Country of Publication: United States
Edition: 1st ed. 2022
Volume: 2529
Dimensions: Height: 254mm, Width: 178mm,
Weight: 1.157kg
ISBN: 9781071624807
ISBN 10: 1071624806
Series: Methods in Molecular Biology
Pages: 495
Publication Date: 24 June 2022
Audience: Professional and scholarly , Undergraduate
Format: Hardback
Publisher's Status: Active