Back Cover Copy
Volume 1 in this book describes how to determine the activity of different isozymes, allozymes, and families of proteinases to advance the fields of enzymology and molecular evolution, and provides useful biomarkers for various biological processes, pathological conditions, and clinical disorders. The chapters in Volume 1 are organized in four parts. Part I introduces gelatin zymography principle, method and precautions, and a practical view of zymography. Part II provides details on the use of zymography to assess various endopeptidases, and includes updates on protease zymography, protocols for gelatin zymography, detection of gelatinases by substrate zymography, unveiling matrix metalloproteinase (MMP) dynamics, and measuring MMPs activity by gelatin zymography. Part III includes chapters on one- and two-dimensional zymography for assessing proteases, loading-controlled zymography, and the use of gel diffusion-based gelatin zymography to analyze MMP-2 and MMP-9 activity in cell culture. Part IV focuses on the use of zymography to assess protease inhibitors and includes chapters that describe polyacrylamide gel electrophoresis as a tool to identify potential inhibitors of protein glycation and analyze glycation-induced protein cross-linking inhibition, kinetics of protease thermal inactivation, and reversible inhibition of cysteine proteases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough, Zymography: Principles, Process, and Pitfalls, Volume I is a valuable resource for both experts in the field, as well as new scientists aspiring to learn and perform successful zymography techniques.
Edited by:
Raouf A. Khalil
Imprint: Springer-Verlag New York Inc.
Country of Publication: United States
Volume: 2917
Dimensions:
Height: 254mm,
Width: 178mm,
ISBN: 9781071644775
ISBN 10: 1071644777
Series: Methods in Molecular Biology
Pages: 270
Publication Date: 11 May 2025
Audience:
Professional and scholarly
,
Undergraduate
Format: Hardback
Publisher's Status: Active
Gelatin Zymography: Principle, Method, and Application in Cancer Research.- Recent Updates on Protease Zymography.- Gelatin Zymography: A Simple and Effective Technique to Detect Gelatinases.- Detection of Gelatinases by Substrate Zymography.- Zymography: Unveiling Matrix Metalloproteinase Dynamics.- Measuring Matrix Metalloproteinases Activity by Gelatin Zymography.- A Practical View of In-Gel Gelatin Zymography of Matrix Metalloproteinases 2 and 9 in Cardiac Tissue.- Gel Zymography for the Evaluation of Matrix Metalloproteinase-2 and -9 in Aorta and Kidney Tissues.- Analysis of Enzymatic Activity of Matrix Metalloproteinases by Collagen Zymography Utilizing Extracted Collagen from Bovine Muscle Tissue.- Electrophoretic Zymography of Protease using Azocasein.- Advanced Zymographic Techniques for Detection and In-Depth Characterization of Microbial Proteases in Enzymology Research.- In-Gel Activity Assay for Glycosidases.- Uncovering Hyaluronidase Activity Using Hyaluronan Zymography in Biological Samples.- Leveraging Zymography to Discover and Investigate Nuclear Proteases.- An Overview of Chitinases Zymography: Past and Present Methods and Protocols.- One- and Two-Dimensional Zymography Methods for Assessing the Family of Proteases.- Loading-Controlled Zymography.- Gel Diffusion-Based Gelatin Zymography to Analyze MMP-2 and MMP-9 Activity in Cell Culture.- Analysis of Protein Glycation Inhibition using Polyacrylamide Gel Electrophoresis.- Analysis of Glycation-Induced Protein Cross-Linking Inhibition using SDS-Polyacrylamide Gel Electrophoresis.- Kinetics of Protease Thermal Inactivation.- Reversible Inhibition of Cysteine Proteases using S-methyl Methanethiosulfonate.
