Yeast Metabolic Engineering

Methods and Protocols

Valeria Mapelli, Maurizio Bettiga

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English

Springer-Verlag New York Inc.
04 July 2022

Microscopy; Biotechnology; Testing of materials

Series: Methods in Molecular Biology

Summary
Details

This second edition volume expands on the previous edition with new and updated chapters on the latest developments in the study of yeast within the biotechnology field. The chapters in this book cover topics such as transformation protocols for genetic engineering of Saccaromyces cerevisiae and Komagataella spp.; an overview of selection markers, promoters, and strains used for metabolic engineering of S. cerevisiae, P. pastoris, and Z. bailii; the use of yeast in CRISPR/Cas9 technology; tools to study metabolic pathway in Yarrowia lypolitica; and a discussion on the “universal expression system” that is applied in a broad spectrum of fungal species. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, andtips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Yeast Metabolic Engineering: Methods and Protocols, Second Edition is a valuable resource for researchers and scientists interested in learning more about this important and developing field.

Edited by: Valeria Mapelli, Maurizio Bettiga
Imprint: Springer-Verlag New York Inc.
Country of Publication: United States
Edition: Second Edition 2022
Volume: 2513
Dimensions: Height: 254mm, Width: 178mm,
Weight: 780g
ISBN: 9781071623985
ISBN 10: 1071623982
Series: Methods in Molecular Biology
Pages: 295
Publication Date: 04 July 2022
Audience: Professional and scholarly , Undergraduate
Format: Hardback
Publisher's Status: Active