This volume is intended to provide both fundamental methods for long-standing assays as well as recent advances in the field toward newer pathways. Chapters guide readers through methods tailored to studying prokaryotic topoisomerases including fundamental protocols for purification and basic characterization of prokaryotic enzymes, enzymology examining DNA cleavage, relaxation, decatenation, and binding to more complex assays dissecting cellular function and interactions, and bioinformatics protocol for examining protein sequence interdependencies with application across many different proteins beyond topoisomerases.
Authoritative and cutting-edge, Topoisomerases: Methods and Protocols as a resource both to new investigators and to long-standing researchers in the field of topoisomerases.
Edited by:
Joseph Deweese Imprint: Springer-Verlag New York Inc. Country of Publication: United States [Currently unable to ship to USA: see Shipping Info] Volume: 2928 Dimensions:
Height: 254mm,
Width: 178mm,
ISBN:9781071645499 ISBN 10: 1071645498 Series:Methods in Molecular Biology Pages: 234 Publication Date:16 May 2025 Audience:
Professional and scholarly
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College/higher education
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Undergraduate
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Further / Higher Education
Format:Hardback Publisher's Status: Active