This detailed volume presents methods for investigating mitotic spindle assembly, chromosome segregation, and cytokinesis. Split into six parts, the book examines advanced microscopes, spatiotemporal manipulation of the spindle and target molecules, quantitative live imaging, screening, centrosome manipulation and purification, and spindle positioning in synthetic or complicated contexts. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding know pitfalls.
Authoritative and up-to-date, The Mitotic Spindle: Methods and Protocols serves as an ideal guide for researchers seeking a better system-level understanding of the mitotic spindle.
Chapters 4, 12, 17, and 19 are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
Edited by:
Tomomi Kiyomitsu Imprint: Springer-Verlag New York Inc. Country of Publication: United States Edition: 2025 ed. Volume: 2872 Dimensions:
Height: 254mm,
Width: 178mm,
ISBN:9781071642238 ISBN 10: 1071642235 Series:Methods in Molecular Biology Pages: 288 Publication Date:02 December 2024 Audience:
Professional and scholarly
,
Undergraduate
Format:Hardback Publisher's Status: Active