The rapid development of HPLC instrumentation and technology opens numerous possibilities - and entails new questions. Which column should I choose to obtain best results, which gradient fits to my analytical problem, what are recent and promising trends in detection techniques, what is state of the art regarding LC-MS coupling? All these questions are answered by experts in ten self-contained chapters. Besides these more hardware-related and technical chapters, further related areas of interest are covered: Comparison of recent chromatographic data systems and integration strategies, smart documentation, efficient information search in internet, and tips for a successful FDA inspection. This practical approach offers in a condensed manner recent trends and hints, and will also display the advanced reader mistakes and errors he was not aware of so far.
Edited by:
Stavros Kromidas (Saarbrücken Germany)
Imprint: Blackwell Verlag GmbH
Country of Publication: Germany
Dimensions:
Height: 249mm,
Width: 175mm,
Spine: 23mm
Weight: 939g
ISBN: 9783527336814
ISBN 10: 3527336818
Pages: 357
Publication Date: 25 May 2016
Audience:
Professional and scholarly
,
Undergraduate
Format: Hardback
Publisher's Status: Active
Survey of current LC/MS instruments Pitfalls of LC/MS coupling Ion chromatography as an application example for LC/MS Separation of biomolecules by chromatographic techniques Gradient optimization for RP-HPLC Gradient prediction Optimization strategies for RP-HPLC Integration methods and strategies HPLC software: performance, comparison, developments Intelligent documentation of HPLC data for regulated studies How to survive an FDA inspection Mining the web for HPLC data Selecting and comparing HPLC columns HPLC hardware: recent trends
Stavros Kromidas studied biology and chemistry at the University of Saarbrucken, where in 1983 he obtained his Ph.D. degree on the development of new chiral stationary phases for HPLC. After working for Waters GmbH for five years, he founded NOVIA GmbH, a provider of professional training and consulting in analytical chemistry, serving as the CEO until 2001. Since 2001 he works as an independent consultant for analytical chemistry, based in Saarbrucken (Germany). For more than 20 years he has regularly held lectures and training courses on HPLC, and has authored numerous articles and several books on various aspects of chromatography.
Reviews for The HPLC Expert: Possibilities and Limitations of Modern High Performance Liquid Chromatography
This book offers a unique perspective on HPLC, with a unique selection of topics that are becoming of evengreater importance in the rapidly expanding field of this technique.The review articles, contributed by well-qualified expertsin their fields, provide a comprehensive overview of thehottest tasks in the field of modern HPLC, and represent avaluable source for specialists in this area of separationscience...Interested readers will find quick and reliableguidance to overcome some common mistakes and drawbacksin the practice of HPLC, as well as useful tips and links forefficient information collection. (Analytical and Bioanalytical Chemistry 23/05/2017)