Recombinant Protein Expression in Mammalian Cells

Methods and Protocols

David L. Hacker

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Hardback

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English

Springer-Verlag New York Inc.
27 June 2024

Scientific equipment, experiments & techniques; Biology, life sciences; Proteins

Series: Methods in Molecular Biology

Summary
Details

This fully updated volume explores notable developments in the field of mammalian cell-based recombinant protein production. Beginning with methods for transient recombinant protein production, the book continues with methods for stable cell pool generation, protein production using stable clonal cell lines, as well as high-throughput screening technologies for characterizing transient cell surface protein ectodomain expression and for identifying host genes involved in protein production. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Recombinant Protein Expression in Mammalian Cells: Methods and Protocols, Second Edition serves as an ideal guide for researchers investigatingprotein structure and function and accelerating the discovery of new therapeutic proteins.

Edited by: David L. Hacker
Imprint: Springer-Verlag New York Inc.
Country of Publication: United States
Edition: Second Edition 2024
Volume: 2810
Dimensions: Height: 254mm, Width: 178mm,
ISBN: 9781071638774
ISBN 10: 1071638777
Series: Methods in Molecular Biology
Pages: 357
Publication Date: 27 June 2024
Audience: Professional and scholarly , Undergraduate
Format: Hardback
Publisher's Status: Active