This work emphasizes the principles and rationale underlying recombinant DNA technology rather than the procedures for executing specific protocols. It suggests flexible approaches to resolving particular molecular necessities that are easily adapted to the readers' own applications. The work features summary tables which provide at-a-glance information on applications of recombinant DNA methodologies.
Edited by:
James Greene (Catholic University Washington D.C. USA),
Venigalla B. Rao
Imprint: CRC Press Inc
Country of Publication: United States
Dimensions:
Height: 276mm,
Width: 219mm,
Spine: 41mm
Weight: 1.570kg
ISBN: 9780824799892
ISBN 10: 0824799895
Pages: 762
Publication Date: 25 June 1998
Audience:
College/higher education
,
Professional and scholarly
,
Further / Higher Education
,
Undergraduate
Format: Hardback
Publisher's Status: Active
Why Cloning?, James J. Greene Biochemistry of Nucleic Acids, Paul S. Miller Enzymology of Recombinant DNA, Frank J. Castora Basic Escherichia coli Genetics Useful for Recombinant DNA Techniques, Darren D. Sledjeski and Susan Garges Formulation of Cloning Strategies, John B. Dame Isolation and Purification of Nucleic Acids, James J. Greene Gel Electrophoresis, Southern and Northern Analysis, James J. Greene and Frank J. Castora Hybridization Probes, Frank J. Castora and James J. Greene cDNA Cloning and the Construction of Recombinant DNA, Dante S. Zarlenga Molecular Cloning by Plasmid Vectors, Paulina Balbás and Francisco Bolívar Phage Lambda: Basic Biology and Use as a Vector, Susan Frackman and Michael Feiss Cosmid Cloning, John Collins and Werner Lindenmaier Multipurpose Cloning Vectors, August J. Sick, Joseph M. Fernandez, and Jay M. Short Mammalian Expression Vectors, Ricky R. Hirschhorn and Nava Sarver Approaches to the Expression of Foreign Genes, Allan R. Shatzman Screening for Specific Recombinant Clones, Lucia Irene González-Villaseñor and Mark M. Manak Principles and Applications of the Polymerase Chain Reaction, Garbriela S. Dveksler and Carl W. Dieffenbach DNA Sequencing, Maurice A. Kashdan, Phillip T. Moen, Jr.,Venigalla B. Rao, and Bachtoi S. Rao Site-Directed Mutagenesis, Alfred Johnson and Marvin Reitz
James Greene (Catholic University, Washington D.C., USA)
Reviews for Recombinant DNA Principles and Methodologies
""" …provide[s] clear insights into the biological, chemical, and physical rationale behind common molecular methods used in lab manuals and commericial kits. ""- Trends in Genetics"
