Quantitative Phase Microscopy and Tomography: Techniques using partially spatially coherent monochromatic...

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Dalip Singh Mehta Ankit Butola

Quantitative Phase Microscopy and Tomography

Techniques using partially spatially coherent monochromatic light

Dalip Singh Mehta, Ankit Butola, Veena Singh

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English

Institute of Physics Publishing
28 December 2022

Biomedical engineering; Light (optics); Image processing

Series: IOP Series in Advances in Optics, Photonics and Optoelectronics

Quantitative phase Microscopy (QPM) has become an important imaging technique in biology for investigating cells and tissues. QPM is an optical interference or holographic microscopic technique in which an input light beam is divided into two beams and one passes through the object and the other acts as a reference beam.

This book develops and describes the most advanced QPM techniques and computational imaging techniques using partially spatially coherent monochromatic light rather than highly coherent lasers. Using partially coherent light as a light source instead of a traditional laser has significantly improved QPM imaging results. Imaging techniques that will be discussed include speckle-free QPM both off-axis and common path interferometric configurations, structured illumination phase microscopy (SIPM), chip-based nanoscopy, machine learning, deep learning and artificial intelligence (AI) in phase microscopy and OCT and Multi-spectral and hyper-spectral phase microscopy. Coherent-noise free QPM techniques described in this book leads to an order of magnitude improved spatial phase sensitivity, space-bandwidth product, and high temporal phase stability. The technique was utilized for sperm cells, macrophages, liver sinusoidal endothelial cells, cancer cells and RBCs for precise QPM. We have demonstrated that partially spatially coherent monochromatic light is most suitable source for high precision QPM.

The text is highly useful for biologists, optical engineers, optical scientists, researchers working in QPM and OCT, and graduate and post graduate students.

Key Features

Presents advances in quantitative phase microscopy and applications for quantitative information.

Includes strategies for generating spatially partially coherent monochromatic light.

Presents coherence-artifacts free off-axis and common path QPM configurations and various algorithms, such as, Fourier Transform and Phase Shifting fringe analysis techniques for phase reconstruction.

Describes advances in QPM and optical coherence tomography (OCT), such as, Machine learning, Support Vector Method and Artificial intelligence architecture for phase analysis.

Contains experimental results on the QPM systems with partially spatially coherent light with significant improvement in spatial-phase sensitivity and phase measurement accuracy compared to coherent laser light.

Contains label-free quantitative analysis of various biological cells and tissues, such as, sperm cells, macrophages, liver sinusoidal cells, and cancerous cells.

Contains futuristic speckle-free multi-spectral and hyperspectral QPM techniques.

By: Dalip Singh Mehta, Ankit Butola, Veena Singh
Imprint: Institute of Physics Publishing
Country of Publication: United Kingdom
Dimensions: Height: 254mm, Width: 178mm, Spine: 14mm
ISBN: 9780750339858
ISBN 10: 0750339853
Series: IOP Series in Advances in Optics, Photonics and Optoelectronics
Pages: 172
Publication Date: 28 December 2022
Audience: Professional and scholarly , Undergraduate
Format: Hardback
Publisher's Status: Active

Dr. Dalip Singh Mehta is currently a Professor at the Department of Physics, Indian Institute of Technology Delhi. He has been actively working in the areas of Bio-photonics (Quantitative Phase Microscopy, Optical Coherence Tomography, Fluorescence Microscopy, White Light Interference Microscopy, Optical Multimodal Techniques for Disease Detection, and Optical Tweezers), Green Photonics: Laser Based Solid State Lighting, Sunlight Harvesting, Optics of LEDs and OLEDs and Optical Metrology. Dr. Ankit Butola received his Ph.D. from the Indian Institute of Technology Delhi. He was a Senior Project Scientist at Indian Institute of Technology Delhi and is currently a Postdoctoral researcher at UiT The Arctic University of Norway. His current research interests are Quantitative Phase Microscopy, Optical Coherence Tomography, Super-resolution Optical Imaging and Computational imaging Techniques. Dr. Veena Singh received her Ph.D. from the Indian Institute of Technology Delhi, India. She is currently Scientist under BIRAC project funded by Department of Biotechnology, Govt. of India and working on point-of-care devices for Oral Breast Cancer screening and diagnostics.