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English
Academic Press Inc
22 October 2024
Protocols in Biochemistry and Clinical Biochemistry, second edition, offers clear, applied instruction in fundamental biochemistry methods and protocols, from buffer preparation to nucleic acid purification, protein, lipid, carbohydrate, and enzyme testing, and clinical testing of vitamins, glucose, and cholesterol levels, among other diagnostics. Each protocol is illustrated with step-by-step instructions, labeled diagrams, and color images, as well as a thorough overview of materials and equipment, precursor techniques, safety considerations and standards, analysis and statistics, alternative methods, and troubleshooting, all to support a range of study types and clinical diagnostics.

This fully revised edition has been expanded and enriched to feature 100 protocols, as well as chapter key term definitions and worked examples. All-new protocols added to this edition include identification of lipids by TLC, lipid per oxidation measurement by thiobarbituric acid assays, determination of serum amylase, catalase activity assay, superoxide dismutase assay, qualitative analysis of plant secondary metabolites, qualitative analysis of photochemicals, quantitative estimation of secondary metabolites, estimation of chlorophyll contents, and starch determination, among others. Each protocol is written to help researchers and clinicians easily reproduce lab methods and ensure accurate test results.
By:   , , , , , , , , , ,
Imprint:   Academic Press Inc
Country of Publication:   United States
Edition:   2nd edition
Dimensions:   Height: 235mm,  Width: 191mm, 
Weight:   450g
ISBN:   9780443139451
ISBN 10:   0443139458
Pages:   264
Publication Date:  
Audience:   Professional and scholarly ,  Undergraduate
Format:   Paperback
Publisher's Status:   Active

Dr. Buddhi Prakash Jain is an Assistant Professor at the Department of Zoology, Mahatma Gandhi Central University, Motihari, Bihar, India. He completed his doctoral studies at the School of Life Sciences, Jawaharlal Nehru University (JNU) New Delhi, India. His research interests include gene expression regulation and endoplasmic reticulum stress in cancer. He is the recipient of several awards including a gold medal in Master Programme (M.Sc.), EMBO travel award, DST (Department of Science and Technology) Govt of India, Young Scientist travel award, and has published several papers in reputed international journals and a number of books and book chapters. Shweta Pandey is an Assistant Professor at the Department of Biotechnology, Govt VYT PG Autonomous College, Durg Chhatisgarh, India. Dr. Pandey received a PhD from the School of Life Sciences, Jawaharlal Nehru University (JNU) New Delhi, India. She has vast teaching experience and has published several papers in reputed international journals, many book chapters in edited books, and co-edited Protocols in Biochemistry and Clinical Biochemistry (Elsevier, 2020). Her research interests include cell cycle regulation, molecular and cell mechanisms of tumor cells, and cancer biology. Shyamal Goswami has a research career spanning 35 years. He has experience teaching in biochemistry, cellular and molecular biology and redox biology at the postgraduate level. He served as Associate Professor and Professor in the School of Life Sciences at Jawaharlal Nehru University, India, from 1998 to 2020. Prior to this he was a Research Assistant Professor in the Department of Anatomy and Cell Biology at the State University of New York-Health Science Centre at Brooklyn, New York, USA. Dr. Goswami has contributed numerous publications across his field and has more than 1500 citations. He also co-authored Protocols in Biochemistry and Clinical Biochemistry and co-edited Post-transcriptional Gene Regulation in Human Disease, both published by Academic Press/Elsevier. His main areas of research are cell signaling and gene expression in the cardiovascular system; redox biology of degenerative diseases; and signal scaffolds and cell functions.

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