This detailed new edition presents the latest developments of the main pillars of protein analysis, namely sample preparation, separation, and characterization. Core areas in this volume are protocols for the analysis of post-translational modifications and protein interaction partners, followed by sophisticated procedures to enrich for extracellular vesicles and organelles, along with several types of protein immuno-assays complemented by various methods for the characterization of antibodies and host-cell protein analysis. Last but not least, a few standard sample preparation protocols and recent advances concerning immuno-chemical detection of proteins are included as well. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Proteomic Profiling: Methods and Protocols, Second Edition serves as an ideal reference for students of biochemistry, biomedicine, biology, and genomics and will be an invaluable source for the experienced, practicing scientist as well.
Edited by:
Anton Posch Imprint: Springer-Verlag New York Inc. Country of Publication: United States Edition: Second Edition 2021 Volume: 2261 Dimensions:
Height: 254mm,
Width: 178mm,
Weight: 1.324kg ISBN:9781071611852 ISBN 10: 1071611852 Series:Methods in Molecular Biology Pages: 592 Publication Date:10 January 2021 Audience:
Professional and scholarly
,
Undergraduate
Format:Hardback Publisher's Status: Active