Protein Methods

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Daniel M. Bollag (Merck Research Laboratories, West Point, Pennsylvania) Michael D. Rozycki (Princeton University, New Jersey)

Protein Methods

Daniel M. Bollag (Merck Research Laboratories, West Point, Pennsylvania), Michael D. Rozycki (Princeton University, New Jersey), Stuart J. Edelstein (University of Geneva, Switzerland) , Michael D. Rozycki

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English

Wiley-Liss Inc.,U.S.
01 August 1996

Scientific equipment, experiments & techniques; Proteins

Protein Methods

Daniel M. Bollag/ Michael D. Rozycki /Stuart J. Edelstein

Reviews from the first edition

""...it is very well written...would certainly be of use to undergraduate and postgraduate students entering the complex world of protein preparation and to more experienced scientists entering this field for the first time.""-Biotechnology and Applied Biochemistry

""The book presents these topics with loads of practical detail, so that the reader has little need to consult other reference sources to carry out the techniques described. All in all, a useful book..."" -Theoretical & Applied Genetics

""...clearly written with protocols that are easy to follow and the text is well spread-out and easy to read...all methods are fully referenced...a useful book for beginners at a reasonable price."" -FEBS Letters

This revised and expanded Second Edition of Protein Methods remains the first source for a complete summary of tested and proven protein techniques. Now divided into two parts, the book begins with the essential chapters from the first edition, updated to reflect important changes in methodology. The chapter on protein isolation includes a new section focusing on the isolation of proteins from inclusion bodies. In the second section, four new chapters are devoted to protein purification and crystallization.

Chapters include
* Preparation for Protein Isolation
* Protein Extraction and Solubilization
* Protein Concentration Determination
* Concentrating Protein Solutions
* Gel Electrophoresis Under Denaturing Conditions
* Gel Electrophoresis Under

...and feature
* reagent items and equipment required for each method
* chapter ""view-at-a-glance"" tabs
* illustrations for all gel applications and other methods
* complete supplier list
* appendices of basic chemical parameters and molecular data
* key references
* descriptive tables
* Safety notes.

By: Daniel M. Bollag (Merck Research Laboratories West Point Pennsylvania), Michael D. Rozycki (Princeton University, New Jersey), Stuart J. Edelstein (University of Geneva, Switzerland), Michael D. Rozycki
Imprint: Wiley-Liss Inc.,U.S.
Country of Publication: United States
Edition: 2nd edition
Dimensions: Height: 257mm, Width: 178mm, Spine: 25mm
Weight: 751g
ISBN: 9780471118374
ISBN 10: 0471118370
Pages: 432
Publication Date: 01 August 1996
Audience: Professional and scholarly , Undergraduate
Format: Paperback
Publisher's Status: Active

Preface xiii Preparation for Protein Isolation 1 Protein Extraction and Solubilization 27 Protein Concentration Determination 57 Concentrating Protein Solutions 83 Gel Electrophoresis Under Denaturing Conditions 107 Gel Electrophoresis Under Nondenaturing Conditions 155 Isoelectric Focusing and Two-Dimensional Gel Electrophoresis 173 Immunoblotting 195 Ion Exchange Chromatography 231 Gel Filtration Chromatography 271 Affinity Chromatography 301 Hanging Drop Crystallization 353 Appendix 1: Molecular weights of commonly used Chemicals 389 Appendix 2: Molecular weights and Isoelectric Points of Selected Protiens 393 Appendix 3: Ammonium Sulfate Precipitation Table 394 Appendix 4: Spectrophotometer Linearity 397 Appendix 5: Suppliers and Addressed 399 Index 405

Daniel M. Bollag and Michael D. Rozycki are the authors of Protein Methods, 2nd Edition, published by Wiley.