This volume discusses traditional and current techniques that are successfully used to diagnose plant viruses and study molecular plant-virus interactions. The chapters in this book cover topics such as in vivo detection of double-stranded RNA, developing rice mutant using CRISPR-Cas9-based technology, protein-protein interaction assays, purification and transfection of protoplasts, protocols for gene silencing, and transmission electron microscopy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and practical, Plant Virology: Methods and Protocols is a valuable resource for plant pathologists, microbiologists, virologists, graduate students, and teachers who are interested in learning more about the developments in plant virology research.
Edited by:
Aiming Wang, Yi Li Imprint: Springer-Verlag New York Inc. Country of Publication: United States Edition: 2021 ed. Volume: 2400 Dimensions:
Height: 254mm,
Width: 178mm,
Weight: 831g ISBN:9781071618349 ISBN 10: 1071618342 Series:Methods in Molecular Biology Pages: 320 Publication Date:15 December 2021 Audience:
Professional and scholarly
,
College/higher education
,
Undergraduate
,
Further / Higher Education
Format:Hardback Publisher's Status: Active