Plant Fungal Pathogens

Fungal ABC Transporter Deletion and Localization Analysis.- Targeted Gene Replacement in Fungal Pathogens via Agrobacterium tumefaciens Mediated Transformation.- Activity-based Protein Profiling of Infected Plants.- The use of Agroinfiltration for Transient Expression of Plant Resistance and Fungal Effector Proteins in Nicotiana benthamiana Leaves.- Proteomic Techniques for Plant-Fungal Interactions.- Identification of HR-inducing cDNAs from Plant Pathogens via a Gateway®-Compatible Binary Potato Virus X-Expression Vector.- Freeze-Fracture of Infected Plant Leaves in Ethanol for Scanning Electron Microscopic Study of Fungal Pathogens.- Real-time PCR for the Quantification of Fungi in planta.- Using Non-homologous End-joining-deficient Strains for Functional Gene Analyses in Filamentous Fungi.- Atomic Force Microscopy (AFM): A Tool for Studying Biophysical Surface Properties Underpinning Fungal Interactions with Plants and Substrates.- Use of the Yeast Two-Hybrid System to Identify Targets of Fungal Effectors.- Characterization of Plant – Fungal Interactions Involving Necrotrophic Effector-Producing Plant Pathogens.- Heterologous Production of Fungal Effectors in Pichia pastoris.- The Application of Laser Microdissection to Profiling Fungal Pathogen Gene Expression in planta.- Metabolomics Protocols for Filamentous Fungi.- Targeted Gene Replacement in Fungi Using a Split-Marker Approach.- Massively Parallel Sequencing Technology in Pathogenic Microbes.- Confocal Microscopy in Plant Pathogen Interactions.- The use of Open Source Bioinformatics Tools to Dissect Transcriptomic Data.- Population Biology of Fungal Plant Pathogens.- Polyethylene Glycol (PEG)-mediated Transformation in Filamentous Fungal Pathogens.- In vitro Induction of Infection-related Hyphal Structures in Plant Pathogenic Fungi.- Fungicide Resistance Assays for Fungal Plant Pathogens.- Identification ofLipid-binding Effectors.- In silico Identification and Characterization of Effector Catalogs.- Horizontal Transfer of Supernumerary Chromosomes in Fungi.- The Induction of Mycotoxins by Trichothecene Producing Fusarium Species.- Identifying Genes in Fusarium verticillioides Through Forward and Reverse Genetics.- Assessment of Autophagosome Formation by Transmission Electron Microscopy (TEM).- Fungal Plant Pathogen Detection in Plant and Soil Samples using DNA Macroarrays.- Random Insertional Mutagenesis in Fungal Genomes to Identify Virulence Factors.- A Yeast-Secretion Trap Assay for Identification of Secreted Proteins from Eukaryotic Phytopathogens and their Plant Hosts.- Comparing Fungal Genomes: Insight into Functional and Evolutionary Processes.- Multi-gene Phylogenetic Analyses to Delimit New Species in Fungal Plant Pathogens.- MAP Kinase Phosphorylation and cAMP Assessment in Fungi.- A One-step Affinity-purification Protocol to Purify NB-LRR Immune Receptors from Plants that Mediate Resistance to Fungal Pathogens.- Karyotyping Methods for Fungi.- Isolation of Apoplastic Fluid from Leaf Tissue by the Vacuum Infiltration-Centrifugation Technique.- Gene Cloning using Degenerate Primers and Genome Walking.- Construction of Hairpin RNA Expressing Vectors for RNA-mediated Gene Silencing in Fungi.- An Unbiased Method for the Quantitation of Disease Phenotypes using a Custom-built Macro Plugin for the Program ImageJ.