Phage Display In Biotechnology and Drug Discovery

Sachdev S. Sidhu, Clarence Ronald Geyer

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English

CRC Press Inc
16 March 2015

Pharmacology; Bacteriology (non-medical); Pharmaceutical technology

Series: Drug Discovery Series

Phage Display in Biotechnology and Drug Discovery, Second Edition provides a comprehensive view of the impact and promise of phage display in drug discovery and biotechnology. Building on the success of its previous edition, the book discusses current theories, principles, and methods in the field and demonstrates applications for peptide phage display, protein phage display, and the development of novel antibodies.

The book provides readers with an overview of the amazing breadth of the impact that phage display technology has had on the study of proteins in general as well as the development of proteins. It will be a valuable resource for those interested in using phage display and recombinant antibodies in basic research and drug discovery.

Edited by: Sachdev S. Sidhu, Clarence Ronald Geyer
Imprint: CRC Press Inc
Country of Publication: United States
Edition: 2nd edition
Volume: 14
Dimensions: Height: 234mm, Width: 156mm, Spine: 34mm
Weight: 1.240kg
ISBN: 9781439836491
ISBN 10: 1439836493
Series: Drug Discovery Series
Pages: 584
Publication Date: 16 March 2015
Audience: College/higher education , Professional and scholarly , Further / Higher Education , Undergraduate
Format: Hardback
Publisher's Status: Active

Filamentous Bacteriophage Structure and Biology. Vectors and Modes of Display. Methods for the Construction of Phage-Displayed Libraries. Selection and Screening Strategies. Leveraging Synthetic Phage-Antibody Libraries for Panning on the Mammalian Cell Surface. Phage Libraries for Developing Antibody-Targeted Diagnostics and Vaccines. Exploring Protein-Protein Interactions Using Peptide Libraries Displayed on Phage. Substrate Phage Display. Mapping Intracellular Protein Networks. High-Throughput and High-Content Screening Using Peptides. Engineering Protein Folding and Stability. Identification of Natural Protein-Protein Interactions with cDNA Libraries. Mapping Protein Functional Epitopes. Selections for Enzymatic Catalysts. Antibody Humanization and Affinity Maturation Using Phage Display. Antibody Libraries from Immunized Repertoires. Naive Antibody Libraries from Natural Repertoires. Synthetic Antibody Libraries. Engineering Antibody Fragments for Intracellular Applications.

Edited by Sachdev S. Sidhu, PhD, professor, Department of Molecular Genetics, University of Toronto, Ontario, Canada C. Ronald Geyer, PhD, professor, Department of Pathology, University of Saskatchewan, Canada

Reviews for Phage Display In Biotechnology and Drug Discovery

... The book covers broadly all aspects of phage-display technology and will be highly appreciated by those working with this technique and interested in applications. ... The book is a clear invitation to use the phage-display technique both in biotechnology and drug discovery; ... this is a very comprehensive book demonstrating the versatility and the value of phage display in many applications. It is clearly written giving an interesting historical background to many presently used variations on the phage-display technique. -Wim Quax, Rijksuniversiteit Groningen, The Netherlands, in ChemBioChem, Vol. 7, No. 699 - 703