Optical Imaging Techniques in Cell Biology

Guy Cox

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Hardback

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English

CRC Press Inc
04 June 2012

Microscopy; Cellular biology (cytology)

A reference on optical imaging techniques, this book covers the field from the basics of the optical microscope to highly advanced techniques such as Forster resonant energy transfer (FRET) and fluorescence lifetime imaging microscopy (FLIM), in a uniform style and format. It provides an understanding of the underlying principles, so readers can understand the benefits and limitations of each technique described. Topics covered include: optical contrasting techniques, fluorescence and fluorescence microscopes, three-dimensional imaging, the confocal microscope, non-linear microscopy, advanced fluorescence techniques, and breaking the diffraction limit. Chapters include suggestions for further reading.

By: Guy Cox
Imprint: CRC Press Inc
Country of Publication: United States
Edition: 2nd edition
Dimensions: Height: 234mm, Width: 156mm, Spine: 18mm
Weight: 680g
ISBN: 9781439848258
ISBN 10: 1439848254
Pages: 316
Publication Date: 04 June 2012
Audience: College/higher education , Further / Higher Education
Format: Hardback
Publisher's Status: Active

The Light Microscope. Optical Contrasting Techniques. Fluorescence and Fluorescence Microscopy. Image Capture. The Confocal Microscope. The Digital Image. Aberrations and Their Consequences. Nonlinear Microscopy. High-Speed Confocal Microscopy. Deconvolution and Image Processing. Three-Dimensional Imaging: Stereoscopy and Reconstruction. Green Fluorescent Protein. Fluorescent Staining. Quantitative Fluorescence. Advanced Fluorescence Techniques: FLIM, FRET, and FCS. Evanescent Wave Microscopy. Beyond the Diffraction Limit. Appendix A: Microscope Care and Maintenance. Appendix B: Keeping Cells Alive under the Microscope. Appendix C: Antibody Labeling of Plant and Animal Cells: Tips and Sample Schedules. Appendix D: Image Processing with ImageJ.

Guy Cox is a professor within the Electron Microscopy Unit at the University of Sydney, Australia.

Reviews for Optical Imaging Techniques in Cell Biology

Praise for the First Edition ...represents an excellent resource for those wishing to gain a grounding in a broad range of optical techniques...written in a highly knowledgeable, enthusiastic and accessible manner...comprehensively covers virtually the entire field of microscopy. ...a valuable addition to the bookshelf of many research laboratories...can quickly and easily provide a clear understanding of commonly used techniques and underlying concepts. Students, technicians, and researchers will find it useful whether they are intending to use the techniques, have been using the techniques for some time, or are merely curious to know more about what the techniques can offer the cell biologist. -Mark Prescott, Department of Biochemistry and Molecular Biology, Monash University, in Australian Biochemist, vol 38 no 3