Monitoring Vesicular Trafficking in Cellular Responses to Stress - Part B, Volume 165 in the Methods in Cell Biology series, highlights new advances in the field, with this new release presenting coverage of different topics, including A novel tool for detecting lysosomal membrane permeabilization by high-throughput fluorescence microscopy, Exploring selective autophagy in Drosophila, Assessment of EGFP-Q74 degradation for the measurement of autophagic flux, Multimodal assessment of autophagy in mammalian cells with a novel, LC3-based tandem reporter, Multiplex quantification of autophagic flux by imaging flow cytometry, Monitoring autophagic flux in Caenorhabditis elegans using p62/SQST-1 reporters, High throughput screening for autophagy, and much more.
1. A novel tool for detecting lysosomal membrane permeabilization by high-throughput fluorescence microscopy Karla Alvarez-Valadez, Allan Sauvat, Helene Fohrer-Ting, Christophe Klein, Oliver Kepp, Guido Kroemer, Mojgan Djavaheri-Mergny 2. Exploring selective autophagy in Drosophila Stavroula Petridi, Anne-Claire Jacomin, Zambarlal Bhujabal, Terje Johansen and Ioannis P. Nezis 3. Assessment of EGFP-Q74 degradation for the measurement of autophagic fluxYan Wang, Jiao Meng, Qi Wu, Guo Chen, Wei Xie, Zhen Zhang, Guido Kroemer and Oliver Kepp 4. Multimodal assessment of autophagy in mammalian cells with a novel, LC3-based tandem reporter Dan F. Lazar, Amani A. Gillette, Samantha R. Lewis, and Braeden L. Butler 5. Multiplex quantification of autophagic flux by imaging flow cytometry Léa Montegut, Hui Chen, Gerasimos Anagnostopoulos, Sabrina Spaggiari, Oliver Kepp, Maria Chiara Maiuri, Guido Kroemer, and Isabelle Martins 6. Monitoring autophagic flux in Caenorhabditis elegans using p62/SQST-1 reporters Christina Ploumi, Aggeliki Sotiriou and Nektarios Tavernarakis 7. High throughput screening for autophagy Sabrina Forveille, Marion Leduc, Allan Sauvat, Giulia Cerrato, Guido Kroemer and Oliver Kepp 8. Determination of mitophagy by electron microscope Zhiyu Li, Qi Wu, Le Liu, Si Sun, Shengrong Sun,Zhong Wang, Juanjuan Li 9. Quantification of intracellular ACBP/DBI levels Valentina Sica, Isabelle Martins, Federico Pietrocola and José Manuel Bravo-San Pedro 10. Immunoblot-based assays for assessing autophagy in the turquoise killifish Nothobranchius furzeri Gerasimos Anagnostopoulos, Leìa Montegut, Hui Chen, Vincent Carbonnier, Isabelle Martins, Khady Mangane, Abdelkrim Mannioui, Alex Bois, Elisa Elena Baracco, Maria Chiara Maiuri, and Guido Kroemer 11. Monitoring autophagy using super-resolution structured illumination and direct stochastic optical reconstruction microscopy Dumisile Lumkwana, Lize Engelbrecht and Ben Loos 12. Monitoring selective autophagy of mitochondria using super-resolution microscopy Ziyue Li and Nicholas T. Ktistakis 13. Monitoring autophagy-dependent ferroptosis Jingbo Li, Rui Kang, and Daolin Tang 14. Quantitative analysis of autophagy-related protein LC3B by quantum-dot-based molecular imaging Xin Yu, Juan Wu, Qi Wu, Shengrong Sun 15. Isolation of microRNA from exosomes Sheila Spada 16. Simultaneous determination of intraluminal lysosomal calcium and pH by dextran-conjugated fluorescence dyes Philippe Pihán, Paula Nunes-Hasler, Nicolas Demaurex and Claudio Hetz
Dr. Oliver Kepp graduated from Humboldt University Berlin and obtained a PhD degree in Molecular Biology. Today he is a tenured researcher at INSERM and a Co-director of the BioCell automation platform at GRCC. Oliver and his team investigate several aspects of tumor immunogenicity, focusing on systems cell biology approaches. He is also a co-founder of Samsara therapeutics and was appointed associate director of the European academy of tumor immunology in 2019. Oliver received the prestigious Clarivate Highly Cited Researcher Award four years in a row since 2018 and has recently been ranked among the top biology and biochemistry scientists in France (#5; www.research.com) Lorenzo Galluzzi is Assistant Professor of Cell Biology in Radiation Oncology at the Department of Radiation Oncology of the Weill Cornell Medical College, Honorary Assistant Professor Adjunct with the Department of Dermatology of the Yale School of Medicine, Honorary Associate Professor with the Faculty of Medicine of the University of Paris, and Faculty Member with the Graduate School of Biomedical Sciences and Biotechnology of the University of Ferrara, the Graduate School of Pharmacological Sciences of the University of Padova, and the Graduate School of Network Oncology and Precision Medicine of the University of Rome “La Sapienza. Moreover, he is Associate Director of the European Academy for Tumor Immunology and Founding Member of the European Research Institute for Integrated Cellular Pathology. Galluzzi is best known for major experimental and conceptual contributions to the fields of cell death, autophagy, tumor metabolism and tumor immunology. He has published over 450 articles in international peer-reviewed journals and is the Editor-in-Chief of four journals: OncoImmunology (which he co-founded in 2011), International Review of Cell and Molecular Biology, Methods in Cell biology, and Molecular and Cellular Oncology (which he co-founded in 2013). Additionally, he serves as Founding Editor for Microbial Cell and Cell Stress, and Associate Editor for Cell Death and Disease, Pharmacological Research and iScience.
