Modern Methods of Drug Design and Development, a volume in the Methods in Enzymology series highlights new advances in the field with this new volume presenting interesting chapters on a variety of topics, including Recombinant protein purification for structural and kinetic studies, Steady-state kinetic analysis of reversible enzyme inhibitors, Steady-State Enzyme Kinetics, Analysis of enzyme kinetic data using ICEKAT, NMR techniques in drug discovery, Dynamic simulations and pre-steady state kinetics to guide drug discovery, Design and assay of substrate-product analogues for racemases and epimerases, Sensitive high throughput methods to screen for P450 inhibition: A- MI complex forming drugs, and more.
Other chapters cover Sensitive high throughput methods to screen for P450 inhibition: B-Heme loss causing drugs, Discovery and development of inhibitors of acetyltransferase Eis to combat Mycobacterium tuberculosis, Crystallographic fragment screening in academic cancer drug discovery, Fast fragment- and compound-screening pipeline at the Swiss Light Source, Chemical biology, enzymology and drug discovery, PROTACs, Proximity-Induced Pharmacology (PROTACs), and much more.
1. Recombinant protein purification for structural and kinetic studies Matthew Lloyd, Otsile Mojanaga and K Ravi Acharya 2. Steady-state kinetic analysis of reversible enzyme inhibitors Matthew Lloyd 3. Steady-State Enzyme Kinetics Bharath Srinivasan 4. Analysis of enzyme kinetic data using ICEKAT Brian C. Smith 5. NMR techniques in drug discovery Tim Woodman 6. Dynamic simulations and pre-steady state kinetics to guide drug discovery Ken Johnson 7. Design and assay of substrate-product analogues for racemases and epimerases Stephen L. Bearne 8. Sensitive high throughput methods to screen for P450 inhibition: A- MI complex forming drugs Rheem Totah 9. Sensitive high throughput methods to screen for P450 inhibition: B-Heme loss causing drugs Rheem Totah 10. Discovery and development of inhibitors of acetyltransferase Eis to combat Mycobacterium tuberculosis Sylvie Garneau-Tsodikova and Oleg Tsodikov 11. Crystallographic fragment screening in academic cancer drug discovery Jane Endicott, Martin Noble and Mathew Martin 12. Fast fragment- and compound-screening pipeline at the Swiss Light Source Justyna A. Wojdyla, May E. Sharpe and Guenter Fritz 13. Chemical biology, enzymology and drug discovery Kevin Dalby 14. PROTACs Matthias Baud 15. Proximity-Induced Pharmacology (PROTACs) Matthieu Schapira 16. Chemo-enzymatic Labelling with asparaginyl endopeptidase (AEP) Louis Luk 17. Development of 5-Aminolevulinic Acid Prodrugs for Photodynamic Therapy and Photodiagnosis Ian Eggleston 18. Systems biology and single cell approaches Udo Opperman
Dr. Matthew Lloyd is a Senior Lecturer at Department of Pharmacy & Pharmacology, Centre for Biosensors, Bioelectronics and Biodevices (C3Bio) of University of Bath, United Kingdom. He completed his Bachelor of Science at the University of Leicester, and his Doctor of Philosophy at the University of Oxford. His research is concentrated in the areas of chemical biology, enzymology, enzyme kinetics, drug discovery and cancer research.
