Divided into three volumes, Micropropagation of Orchids Third Edition retains the exhaustive list of micropropagation protocols for many genera and updates each section to include new and/or revised information about:
Culture media and vessels Techniques and procedures for both orchids which were previously cultured and for those which were not Plant hormones and growth regulators Media components Methods for tissue decontamination Historical information Procedures for the cultivation for plantlets which have been removed from flasks Sources of light and illumination methods
Written by two globally acknowledged experts in the field, the third edition of this definitive text on the micropropagation of orchids is a detailed and comprehensive collection of procedures and methods for multiplying orchids, including organ, tissue, and cell culture techniques in vitro and is intended for researchers in plant science and propagation, professional and amateur orchid growers, and plant breeding professionals. Much of the general information about techniques and procedures can be applied to plants other than orchids.
Preface Preface to the Second Edition Preface to the First Edition Chapter 1: History Terminology Origins of Orchid Micropropagation Plant Hormones and Propagation Additives of Plant Origin Culture of Tissues and Organs Early Tissue Culture Attempts Culture of Stem Tips First Micropropagation of Orchids The Second Aseptic Culture of an Orchid Explant Plant Diseases and Meristems The Third Aseptic Culture of an Orchid Explant The Fourth Aseptic Culture of an Orchid Explant Who Pioneered Micropropagation? Root Cultures Leaf Cultures Stems Flower Buds, Flowers, Floral Segments, and Reproductive Organs Inflorescences A Patent Doubtful Claims Mutations Theft in Vitro Darkening of Culture Media Anticontaminants Cell and Protoplast Culture Flowering in Vitro, The Future Chapter 2: General Outline of Techniques and Procedures Media Components Macroelements Microelements Iron Auxins and Anti-auxin Chitosan Cytokinins Gibberellins and Antigibberellin Abscisic Acid Ethylene and Ethylene Inhibitor Amino Acids Polyol Polyamines Phloroglucinol Jasmonates Vitamins Nucleotides and Nucleic Acids Organic Acids Banana Coconut Water Hydrolysates and Autolysates Sugars Anticontaminants Charcoal Solvents Solidifiers pH Recipes in this Book Stock Solutions State of the Medium Sterilization Autoclaves Filtration Sterilization in Microwave Ovens Solvents Open Flame Liquids Surface Decontamination Gas Sterilization Preparation of the Medium Culture Vessels Culture Conditions Temperature Agitation Illumination Carbon Dioxide Placing Plant Material in Culture Seeds Tissue Explants Internal Contaminants Enzymes for Protoplast Isolation Work Area Laminar-flow Hoods Sterile Rooms Sterile Boxes Clean Laboratory Bench Washing Glassware Chapter 3: Methods for Specific Genera Acampe Aeridachnis Aerides Aeridovanda Anacamptis Angraecum Anoectochilus Ansellia Arachnis Arachnostylis Aranda Aranthera Arundina Ascocenda Ascocentrum Ascofinetia Barlia Bletilla Brassavola Brassia Brassocattleya Brassolaeliocattleya, 277 Bulbophyllum Burkillara Caladenia Calanthe Catasetum Cattleya Caularthron Cleisostoma Clowesia Coelogyne Cymbidium Cypripedium Cyrtopodium Dactylorchis Dactylorhiza Darwinara Dendrobium, 518 Disa Diuris Doriella Doritaenopsis Doritis, 693 Encyclia Epidendrum Epiphronitis Erycina Eulophia Gastrochilus Geodorum Goodyera Grammatophyllum Habenaria Hadrolaelia Hetaeria Holttumara Ipsea Kagawara Laelia Laeliocattleya Liparis Ludisia (Haemaria) Luisia Lycaste Malaxis Maxillaria Miltonia Mokara Mormodes Neofinetia Neostylis Neottia Nervilia Nigritella Oberonia Odontioda Odontoglossum Odontonia Oncidium Ophrys Orchis Ottochilus Pachystoma Paphiopedilum Papilionanda Papilionanthe, 901 Phaius Phalaenopsis Pholidota Phragmipedium Pleione Pogonia Ponerorchis Potinara Pterostylis Renades Renanetia Renantanda Renanthera Restrepia Rhynchostele Rhynchostylis Saccolabium Sarcanthus Satyrium Schomburgkia Serapias Sophrolaeliocattleya Spathoglottis Spiranthes Tainia Thelymitra Thunia Trudelia Vanda Vandofinetia Vanilla Vascostylis Vuylstekeara, 1292 Zygopetalum, 1292 Chapter 4: Summary Culture Conditions Media Components Macroelements Microelements Substitutions Carbohydrates Amino Acids Vitamins Polyols Hormones Coconut Water Bleeding Sap of Birch Trees Other Complex Organic Additives Solidifying (Gel-forming) Agents and Alternative Supporting Media Adsorbents Applications of Orchid Tissue Culture Commercialization Conservation Patents Plant Improvement and Biotechnology Cryopreservation Physiological Studies Negative Aspects of Micropropagation References Appendix 1: General Information on Supplies, Equipment, Terms, and Reagents Appendix 2: Sources of Supplies and Equipment Appendix 3: Some Sites of Interest on the World Wide Web Appendix 4: Light Appendix 5: Formulary Appendix 6: Atomic Weights, Concentrations, Exponents, Greek and Roman Letters, Ions, Measurements, Molecular Weights, Prefixes, Valences, Units, and Solutions Appendix 7: Additional Information Appendix 8: Plant Preservative Mixture Appendix 9: Media Appendix 10: ASCII Codes Glossary Index
Tim Wing Yam, Singapore Botanic Gardens, National Parks Board, Singapore Joseph Arditti, Professor Emeritus, University of California, Irvine, USA
