A Step-by-Step Guide to Present and Future Uses of Microarray Technology
Microarray technology continues to evolve, taking on a variety of forms. From the spotting of cDNA and the in situ synthesis of oligonucleotide arrays now come microarrays comprising proteins, carbohydrates, drugs, tissues, and cells. With contributions from microarray experts in both academia and industry, Microarray Methods and Protocols is a turn-by-turn roadmap through the processes necessary to perform a successful microarray experiment.
This easy to use book addresses the fundamental aspects of preparing and processing microarrays and bead arrays, labeling, and detection. It also includes a detailed How it Works section that discusses the underlying principles of a number of techniques. Troubleshooting guides offer additional advice for the successful performance of more than 100 protocols in 10 chapters that cover work involving nucleic acids, proteins, carbohydrates, and lectins. --Concise and Well-Organized--
With a focus on the preparation and use of microarrays of biomedical relevance, the text describes a variety of microarray formats useful in the assessment of human disease and in genomic and proteomic research. This authoritative resource provides detailed information regarding sample preparation, labeling, array construction processes, substrate chemistry, array printing, and quality control. Originating with the glass microscope slide and biochip, microarray technology is now pressing onward into the nanotechnology frontier. This book is the all-inclusive manual scientists need to take microarray research to the next level of discovery.
By:
Robert S. Matson (QuantiScientifics LLC Irvine California USA)
Imprint: CRC Press Inc
Country of Publication: United States
Dimensions:
Height: 234mm,
Width: 156mm,
Spine: 15mm
Weight: 408g
ISBN: 9781420046656
ISBN 10: 1420046659
Pages: 246
Publication Date: 20 January 2009
Audience:
Professional and scholarly
,
Undergraduate
Format: Paperback
Publisher's Status: Active
Introduction to Microarray Technologies. Nucleic Acid Sample Preparation. Solid-Phase Substrates for Nucleic Acid Microarrays. Protein Sample Preparation for Microarrays. Solid-Phase Chemistries for Protein Microarrays. Protein Microarrays: The Link between Genomics and Proteomics. Bead Arrays: An Introduction to Multiplexed Bead-Based Assays for Proteins. Carbohydrate Arrays. Lectin Microarrays. Printing Methods. Appendix A: Microarray Reagent, Materials, and Equipment Sources. Appendix B: Image Analysis. Index.
Beckman Coulter Inc., Fullerton, California, USA
Reviews for Microarray Methods and Protocols
The book will be useful for researchers in protein chemistry and biochemistry, molecular biologists, and teachers and students of medical schools and universities specializing in microarray methods. --Doctor of Biological Sciences, G. Ya. Wiederschain. Biochemsitry - Moscow. 2010, VOL 75, No 9,1182
