Maternal Placental Interface

Methods and Protocols

Verónica Zaga-Clavellina

$370.95 $296.98

Hardback

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English

Springer-Verlag New York Inc.
20 March 2024

Medical research; Reproductive medicine; Molecular biology

Series: Methods in Molecular Biology

This volume details critical and valuable experimental protocols to facilitate researchers in this fascinating field. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Maternal Placental Interface: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Edited by: Verónica Zaga-Clavellina
Imprint: Springer-Verlag New York Inc.
Country of Publication: United States
Edition: 2024 ed.
Volume: 2781
Dimensions: Height: 254mm, Width: 178mm,
ISBN: 9781071637456
ISBN 10: 1071637452
Series: Methods in Molecular Biology
Pages: 192
Publication Date: 20 March 2024
Audience: Professional and scholarly , Undergraduate
Format: Hardback
Publisher's Status: Active

Histological analysis of trophoblast cells in the mouse placental labyrinth zone.- Isolation and Immunophenotyping of Leukocytes from the Human Maternal-Fetal Interface.- Evaluation of leukocyte chemotaxis induced by human fetal membranes in an in vitro model.- In vitro culturing of Human Term Placental Explants.- In vitro culturing of Human Trophoblasts from Term Placenta.- Culture of human fetal membranes in a two independent-compartment model: an ex vivo approach.- Isolation of Primary Human Decidual Cells from the Fetal Membranes of Term Placentae.- Generation of knockout mouse trophoblast stem cells by CRISPR/Cas.- CRISPR-activation in mouse trophoblast stem cells.- Feto-maternal interface organ-on-chip: a new technology to study ascending infection.- Culture and Maintenance of Immune Cells to Model Innate Immune Status at the Feto-Maternal Interface.- Placental trophoblast cell isolation from the term placenta.- Dissociation of placental tissues for single-cell techniques.- Immunophenotyping of leukocytes in amniotic fluid.- Obtainig of tissue of human amniotic membrane and identification of pluripotency markers by immunofluorescence.- DNA isolation from fresh placenta, in formalin and paraffin.- Infection of fetal membranes with Mycobacterium tuberculosis and tissue processing to isolate RNA for expression analysis.

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Maternal Placental Interface

Methods and Protocols

Verónica Zaga-Clavellina

$370.95 $296.98

Hardback

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