Mass Spectrometry for Lipidomics All-in-one guide to successful lipidomic analysis, combining the latest advances and best practices from academia, industry, and clinical research
Mass Spectrometry for Lipidomics presents a systematic overview of lipidomic analysis, covering established standards of lipid analysis, available technology, and key lipid classes, as well as applications in basic research, medicine, pharma, and the food industry.
Through connecting recent technological advances with key application areas, this unique guide bridges the gap between academia and industry by translating the vast body of knowledge that has been gained in the past decade into much-needed practical advice for novices as well as routine users.
Edited by the president and vice-president of the International Lipidomics Society with contributions from the top experts in lipid analysis, Mass Spectrometry for Lipidomics covers a wide range of key topics, including:
Aspects of sample preparation, separation methods, different mass spectrometry modes, as well as identification and quantitation, including the use of bioinformatics tools for data analysis
Identification, quantitation and profiling of lipids in different types of biological samples
Analytical approaches for all major classes of biological lipids, from fatty acids to phospholipids to sterols
Novel applications in biological research, clinical diagnostics, as well as food and crop science
For analytical chemists, biochemists, clinical chemists, and analytical laboratories and hospitals, Mass Spectrometry for Lipidomics presents a comprehensive and authoritative overview of the subject, with unmatched expertise from practicing professionals actively involved in the latest research.
Preface xiii 1 Introduction to Lipidomics 1 Harald C. Köfeler, Kim Ekroos, and Michal Holcapek Part I Analytical Methodologies in Lipidomics 13 2 Preanalytics for Lipidomics Analysis 15 Gonçalo Vale and Jeffrey G. McDonald 3 Direct Infusion (Shotgun) Electrospray Mass Spectrometry 41 Marcus Höring and Gerhard Liebisch 4 Liquid Chromatography – and Supercritical Fluid Chromatography – Mass Spectrometry 91 Michal Holcapek, Ondrej Peterka, Michaela Chocholousková, and Denise Wolrab 5 Mass Spectrometry Imaging of Lipids 117 Shane R. Ellis and Jens Soltwisch 6 Ion Mobility Spectrometry 151 Kaylie I. Kirkwood, Melanie T. Odenkirk, and Erin S. Baker 7 Structural Characterization of Lipids Using Advanced Mass Spectrometry Approaches 183 Josef Cvacka, Vladimír Vrkoslav, and stepán Strnad 8 Lipidomic Identification 227 Harald Köfeler 9 Lipidomics Quantitation 255 Michaela Chocholousková, Denise Wolrab, Ondrej Peterka, Robert Jirásko, and Michal Holcapek 10 The Past and Future of Lipidomics Bioinformatics 271 Dominik Kopczynski, Daniel Krause, Fadi Al Machot, Dominik Schwudke, Nils Hoffmann, and Robert Ahrends Part II Lipidomic Analysis According to Lipid Categories and Classes 291 11 Fatty Acids: Structural and Quantitative Analysis 293 Dong Hao Wang and J. Thomas Brenna 12 Quantitation of Oxylipins in Biological Samples, Focusing on Plasma, and Urine 317 Valerie B. O'Donnell, Ginger L. Milne, Marina S. Nogueira, Martin Giera, and Nils Helge Schebb 13 Mass Spectrometry for Analysis of Glycerolipids 351 Wm. Craig Byrdwell 14 Lipidomic Analysis of Glycerophospholipid Molecular Species in Biological Samples 395 Xianlin Han 15 Sphingolipids 425 Lukas Opalka, Lisa Schlicker, and Roger Sandhoff 16 Sterol Lipids 481 William J. Griffiths, Eylan Yutuc, and Yuqin Wang 17 Bile Acids 509 Sebastian Simstich and Günter Fauler Part III Lipidomic Applications 531 18 Lipidomic Profiling in a Large-Scale Cohort 533 Daisuke Saigusa 19 Cancer Lipidomics – From the Perspective of Analytical Chemists 545 Denise Wolrab, Ondrej Peterka, Michaela Chocholousková, and Michal Holcapek 20 Lipidomics in Clinical Diagnostics 557 Jayashree Selvalatchmanan, Markus R. Wenk, and Anne K. Bendt 21 Lipidomics in Food Industry and Nutrition 585 Danilo Donnarumma, Giuseppe Micalizzi, Luigi Mondello, and Paola Dugo 22 Lipidomics in Plant Science 601 Zoong Lwe Zolian, Yu Song, P. A. D. B. Vinusha Wickramasinghe, and Ruth Welti 23 Lipidomics in Multi-Omics Studies 625 Bjoern Titz, Oksana Lavrynenko, and Nikolai V. Ivanov 24 Tracer Lipidomics 641 Jonas Dehairs, Ine Koeken, Lake-Ee Quek, Andrew Hoy, Bart Ghesquière, and Johannes V. Swinnen 25 Mass Spectrometry for Lipidomics: Methods and Applications – Aging and Alzheimer's Disease 657 Kevin Huynh, Habtamu B. Beyene, Tingting Wang, Corey Giles, and Peter J. Meikle 26 Lipidomics in Cell Biology 669 Noemi Jiménez-Rojo, Fabrizio Vacca, and Howard Riezman 27 Microbial Lipidomics 689 Masahiro Ueda, Nobuyuki Okahashi, and Makoto Arita Index 705
Michal Holèapek obtained his Ph.D. at the University of Pardubice (Czech Republic), where he is now a full professor of analytical chemistry. His research focus is mass spectrometry and its coupling with liquid chromatography and supercritical fluid chromatography. He is the current vice-president for conferences of the International Lipidomics Society. Kim Ekroos is the founder and CEO of Lipidomics Consulting Ltd. He received his Ph.D. degree in biology from the Technical University in Dresden (Germany). He is the current president of the International Lipidomics Society.
