Manual of Sperm Function Testing in Human Assisted Reproduction

Ashok Agarwal, Ralf Henkel (University of the Western Cape, South Africa), Ahmad Majzoub

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English

Cambridge University Press
15 April 2021

Human reproduction, growth & development; Reproductive medicine; Infertility & fertilization

Selecting good-quality sperm for use in in-vitro fertilization is a key step in assisted reproduction. For many years purely morphological attributes have been used to assess suitability, but increasingly biochemical and molecular biological techniques are now identifying sperm with the best chances of producing viable and healthy embryos. Focusing on modern sperm function testing, this manual provides technical details of commonly used tests and gives an overview of the laboratory techniques used to evaluate sperm samples. Covering a variety of testing methods in detail, from manual and computer-assisted semen analysis to zona pellucida binding assays, and tests assessing sperm DNA damage such as the TUNEL assay. Describing the underlying science, practical advice for performing the tests is given, including tips for optimizing outcomes and trouble-shooting. This is an essential guide for reproductive medicine specialists, clinical andrologists, urologists and gynecologists working with sub-fertile men.

Edited by: Ashok Agarwal, Ralf Henkel (University of the Western Cape, South Africa), Ahmad Majzoub
Imprint: Cambridge University Press
Country of Publication: United Kingdom
Dimensions: Height: 246mm, Width: 189mm, Spine: 15mm
Weight: 610g
ISBN: 9781108793537
ISBN 10: 1108793533
Pages: 282
Publication Date: 15 April 2021
Audience: Professional and scholarly , Undergraduate
Format: Paperback
Publisher's Status: Active

Ashok Agarwal is the Director of the Clinical Andrology Laboratory and Reproductive Tissue Bank and the Director of Research at the American Center for Reproductive Medicine. He holds these positions at The Cleveland Clinic Foundation, where he is a Professor of Surgery (Urology) at the Lerner College of Medicine of Case Western Reserve University, Cleveland, Ohio, USA. Ralf Henkel is the Chief Scientific Advisor for LogixX Pharma Ltd and Editor-in-Chief of Andrologia. He is also an Extraordinary Professor at the University of the Western Cape, Bellville, South Africa and an Honorary Professor at the Universidad Peruana Cayetano Heredia, Lima, Peru, American Center for Reproductive Medicine, Cleveland Clinic, Cleveland, OH. Ahmad Majzoub is a Consultant in the Department of Urology and Program Director of the Andrology Fellowship at the Hamad Medical Corporation, Doha and an Assistant Professor of Clinical Urology at Weill Cornell Medicine, Doha, Qatar.

Reviews for Manual of Sperm Function Testing in Human Assisted Reproduction

'This is truly a compendium of human sperm function assays. The chapters include background information, some detailed protocols, interesting and insightful drawings, and photographs and insights from the authors/creators of the assays ... it should have a spot on the shelves of every biologist with an interest in human sperm function.' Karla Saner Amigh, Doody's Notes