Lymphocytes

A Practical Approach

Rowland-Jones, McMichael, Andrew McMichael

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English

Oxford University Press
01 March 2000

Immunology; Scientific equipment, experiments & techniques; Biochemical immunology

Series: Practical Approach Series

Cellular immunology is a rapidly moving field in which recent advances have made significant contributions to our understanding of the immune response to infection and malignancy.

These in turn, have given rise to new therapeutic opportunities in areas such as vaccines and immunotheraphy.

Many investigators have been discourages by the complicated protocols involved in cellular immunological studies, as illustrated, by the meticulous care required for the generation of antigen-specific T-cells.

Lymphocytes: A Practical Approach (second edition) contains straight-forward protocols for well- established procedures in the study of lymphocytes including preparation and identification of lymphocytes, immortalization, cell and organ culture, and quantification assays. It also covers the recent technological advances which have revolutionised the field, such as the use of the Interferon-gamma ELISpot assay and peptide-HLA tetrameric assays to quantify antigen-specifidc T-cells directly from peripheral blood, without the need for in vitro culture, and molecular methods for accurate HLA typing.

Edited by: Rowland-Jones, McMichael, Andrew McMichael
Imprint: Oxford University Press
Country of Publication: United Kingdom
Edition: 2nd Revised edition
Volume: 220
Dimensions: Height: 234mm, Width: 157mm, Spine: 21mm
Weight: 649g
ISBN: 9780199638161
ISBN 10: 0199638160
Series: Practical Approach Series
Pages: 376
Publication Date: 01 March 2000
Audience: Professional and scholarly , Undergraduate
Format: Paperback
Publisher's Status: Active

Preface Plebanski: Preparation of lymphocytes and idenfication of lymphocyte subpopulations Biddulph and Gatter: Immunohistochemistry of lymphoid organs Meinl and Fickenscher: Immortalisation of lymphocytes Bastin: T and B-cell hybridomas Mills: Murine T-cell culture Vyakarnam: Human CD4+ T-cell culture Rowland-Jones: Human Cytotoxic T-cell culture Carmichael: Limiting dilution analysis for quantification of antigen-specific T-cells Ogg: HLA -peptide tetrameric complexes Tan: Expansion of human T-cells for immunotheraphy Browning and Krausa: HLA typing Brown and Symons: Characterisation of lymphocyte surface markers Bangham and Furukawa: Apoptosis assays for lymphocytes Owen and McLoughlin: Thymic organ culture Index