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English
Academic Press Inc
15 March 2026
Lipids and Membranes: Dynamics and Interorganelle Lipid Transport, Volume 728 covers lipids, a chemically diverse set of biological molecules subject to rapid metabolism and transport. This dynamic behavior has motivated the development of methods across multiple disciplines for their detection and manipulation. Split into three parts, the book's chapters include topics such as Assay of phospholipid scrambling, Tracking lipid transport with bifunctional lipids, Tracking interorganelle lipid transport using genetically encoded mass tags, Molecular Dynamics Simulation of Lipid and Ligand Binding in ORD Domains, Imaging lipid droplet-organelle membrane contact sites using fluorogenic complementation, Studying interactions of amphipathic helices with lipid droplets, and more.

Additional chapters cover Metabolic labeling of to trace changes in sphingolipid metabolism, Imaging intracellular lipid heterogeneity with fluorogen-activated coincidence sensing, Tagging lipoproteins in mycobacteria, Probes for studying sphingolipid dynamics, Measuring Flipper-TR Lifetime with Multiple Analysis Strategies, Reconstitution approaches to understanding cellular lipid dynamics, Prediction and characterization of the bacterial lipid handling machinery, From Molecular Dynamics to Cryo-EM: Imaging Liposomes in Silico, and much more.
Series edited by:   , , , , , ,
Volume editor:  
Imprint:   Academic Press Inc
Country of Publication:   United States
Dimensions:   Height: 229mm,  Width: 152mm, 
Weight:   450g
ISBN:   9780443433146
ISBN 10:   0443433143
Series:   Methods in Enzymology
Pages:   474
Publication Date:  
Audience:   Professional and scholarly ,  College/higher education ,  Undergraduate ,  Further / Higher Education
Format:   Hardback
Publisher's Status:   Active
1. Assay of phospholipid scrambling Anant K. Menon 2. Tracking lipid transport with bifunctional lipids Andre Nadler 3. Tracking interorganelle lipid transport using genetically encoded mass tags Benoit Kornmann 4. Molecular Dynamics Simulation of Lipid and Ligand Binding in ORD Domains Bruno Mesmin 5. Imaging lipid droplet-organelle membrane contact sites using fluorogenic complementation Chi-Lun Chang 6. Studying interactions of amphipathic helices with lipid droplets Fabien Alpy 7. Metabolic labeling of to trace changes in sphingolipid metabolism Fikadu Tafesse 8. Imaging intracellular lipid heterogeneity with fluorogen-activated coincidence sensing Itay Budin 9. Tagging lipoproteins in mycobacteria Jessica Seeliger 10. Probes for studying sphingolipid dynamics Joost Holthuis 11. Measuring Flipper-TR Lifetime with Multiple Analysis Strategies Juan Manuel Garcia-Arcos 12. Reconstitution approaches to understanding cellular lipid dynamics Karin Reinisch 13. Prediction and characterization of the bacterial lipid handling machinery Laura Massah Kulah Dassama 14. From Molecular Dynamics to Cryo-EM: Imaging Liposomes in Silico Milka Doktorova 15. Genetic screens for lipid discovery in Drosophila Raghu Padinjat 16. Imaging interorganelle membrane contact sites using dimerization-dependent fluorescent proteins Sarah Cohen 17. Organelle-selective labeling of lipids containing azide-fatty acids Tomonori Tamura 18. Analysis of interorganelle lipid exchange Vytas Bankaitis 19. Reconstitution of full-length extended synaptotagmin 1 into liposomes for membrane tethering and lipid transfer assays Xin Bian 20. Use of Small Unilamellar Vesicles to Study the Biochemical Properties of the GRAM Domain of GRAMD1 Yasunori Saheki

After completing studies for the A.B., A.M., and Ph.D. degrees in chemistry at Harvard University, David W. Christianson joined the faculty of the University of Pennsylvania, where he is currently the Roy and Diana Vagelos Professor in Chemistry and Chemical Biology. At Penn, Christianson’s research focuses on the structural and chemical biology of the zinc-dependent histone deacetylases as well as enzymes of terpene biosynthesis. His research accomplishments have been recognized by several awards, including the Pfizer Award in Enzyme Chemistry and the Repligen Award in Chemistry of Biological Processes from the American Chemical Society, a Guggenheim Fellowship, and the Elizabeth S. and Richard M. Cashin Fellowship from the Radcliffe Institute for Advanced Study at Harvard University. Christianson is also a dedicated classroom teacher, and his accomplishments in this regard have been recognized by the Lindback Award for Distinguished Teaching at Penn and a Rhodes Trust Inspirational Educator Award from Oxford University. Christianson has also held visiting professorships in the Department of Biochemistry at Cambridge University and the Department of Chemistry and Chemical Biology at Harvard University. Christianson has served with Prof. Anna Pyle as Co-Editor-in-Chief of Methods in Enzymology since 2015. Dr. Karen N. Allen works at the Department of Chemistry of the Boston University, the Metcalf Center for Science and Engineering Jeremy M. Baskin is Associate Professor, Nancy and Peter Meinig Family Investigator in the Life Sciences, and Director of the Chemistry–Biology Interface Program at Cornell University, with appointments in the Department of Chemistry and Chemical Biology and the Weill Institute for Cell and Molecular Biology. He was born and raised in Montreal, Canada and received his undergraduate education at the Massachusetts Institute of Technology, with a major in Chemistry and minors in Biology and Music. Jeremy carried out Ph.D. studies supported by NDSEG and NSF graduate fellowships in Carolyn Bertozzi’s group at the University of California, Berkeley, focusing on development of bioorthogonal chemistries. Jeremy received postdoctoral training in lipid cell biology as a Jane Coffin Childs fellow at Yale University with Pietro De Camilli. Research in the Baskin lab centers on the chemical and cell biology of lipid metabolism and signaling. The Baskin lab exploits bioorthogonal chemistry to develop advanced tools for high-resolution lipid imaging and harnesses optogenetics and protein engineering for the design of membrane editors capable of spatiotemporal manipulation of the lipid composition of cellular membranes. Using these and other approaches, his lab elucidates novel mechanisms underlying physiological and pathological lipid metabolism and signaling events. Jeremy has been the recipient of numerous awards, including Beckman Young Investigator, Sloan Research Fellowship, NSF CAREER, ACS Young Academic Investigator, ASBMB Walter A. Shaw Young Investigator in Lipid Research, ICBS Young Chemical Biologist Award, and ACS Chemical Biology Young Investigator Award. He is currently Associate Editor at Biochemistry.

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