Lipid Rafts

Methods and Protocols

Erhard Bieberich

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English

Springer-Verlag New York Inc.
10 August 2021

Spectrum analysis, spectrochemistry, mass spectrometry; Lipids

Series: Methods in Molecular Biology

Summary
Details

This volume gives a comprehensive insight into established and novel methods to analyze the structure and function of lipid rafts. This book covers topics such as isolation of lipid rafts and their functional analysis using biochemical methods; visualization of lipid rafts and their interaction with proteins using fluorescence-related methods; preparation of giant lipid vesicles and fluorescence spectroscopy; FRET and FRAP; and using photo-activated cross-linking of a ceramide analog combined with proximity ligation assay. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Thorough and cutting-edge, Lipid Rafts: Methods and Protocols is a valuable resource for both novice and expert researchers interested in learning moreabout the function of lipid rafts in many areas of cell biology and medicine.

Edited by: Erhard Bieberich
Imprint: Springer-Verlag New York Inc.
Country of Publication: United States
Edition: 2021 ed.
Volume: 2187
Dimensions: Height: 254mm, Width: 178mm,
Weight: 692g
ISBN: 9781071608166
ISBN 10: 1071608169
Series: Methods in Molecular Biology
Pages: 350
Publication Date: 10 August 2021
Audience: College/higher education , Professional and scholarly , Further / Higher Education , Undergraduate
Format: Paperback
Publisher's Status: Active