Lipids can usually be extracted easily from tissues by making use of their hydrophobic characteristics. However, such extractions yield a complex mixture of different lipid classes which have to be purified further for quantitative analysis. Moreover, the crude lipid extract will be contami nated by other hydrophobic molecules, e.g. by intrinsic membrane proteins. Of the various types of separation processes, thin layer and column chromatography are most useful for intact lipids. High performance liquid chromatography (HPLC) is also rapidly becoming more popular, especially for the fractionation of molecular species of a given lipid class. The most powerful tool for quantitation of the majority of lipids is gas liquid chromatography (GLC). The method is very sensitive and, if adapted with capillary columns, can provide information with regard to such subtle features as the position or configuration of substitutions along acyl chains. By coupling GLC or HPLC to a radioactivity detector, then the techniques are also very useful for metabolic measurements. Although research laboratories use generally sophisticated analytical methods such as GLC to analyse and quantify lipid samples, chemical derivatii:ations are often used in hospitals. For these methods, the lipid samples are derivatized to yield a product which can be measured simply and accurately""- usually by colour. Thus, total triacylglycerol, cholesterol or phospholipid-phosphorus can be quantitated conveniently without bothering with the extra information of molecular species, etc. which might be determined by more thorough analyses. REFERENCES Christie, W.W. (1982) Lipid Analysis, 2nd edn, Pergamon Press, Oxford.
By:
M. Gurr
Imprint: Springer
Edition: Softcover reprint of the original 1st ed. 1991
Dimensions:
Height: 235mm,
Width: 155mm,
Spine: 21mm
Weight: 640g
ISBN: 9789401053600
ISBN 10: 940105360X
Pages: 406
Publication Date: 12 November 2012
Audience:
College/higher education
,
Professional and scholarly
,
Further / Higher Education
,
Undergraduate
Format: Paperback
Publisher's Status: Active
1 The nature of lipids and their place in living things.- 2 Isolation, separation and detection of lipids.- 3 Fatty acid structure and metabolism.- 4 Lipids as energy stores.- 5 Dietary lipids: implications for health and disease.- 6 Lipids in cellular structures.- 7 Metabolism of structural lipids.- 8 Lipid functions.
