Lignin-Degrading Enzymes

1. Lignin characterisation methods for studying enzymatic/microbial lignin degradation Stéphanie Baumberger 2. Identification and quantification of lignin enzymatic oxidation pathways by NMR spectroscopy Claudia Crestini 3. Assay methods and colorimetric screens for lignin degradation activity by enzymes and microbes Timothy David Bugg 4. Use of EPR to study radical formation by lignin-degrading enzymes Anne S. Meyer 5. Use of FTIR to study lignin-degrading enzymes Anne S. Meyer 6. Dye-decolorizing peroxidase DyP1B from Pseudomonas fluorescens and its reaction with polymeric lignin substrates Timothy David Bugg 7. Optimized Protocols to Measure Stability of Ligninolytic Enzymes Ligia O. Martins, Eduardo P. Melo, Carolina F. Rodrigues, Paulo Durao and Vânia Brissos 8. Enzymology of Streptomyces DyP-type peroxidases Hegne Pupart 9. Expression of Streptomyces LAC and dye-decolorizing peroxidases in Streptomyces lividans Clemens K. Peterbauer, Silja Välimets and Jessica Virginia 10. Design, construction and characterization of laccase-xylanase chimeras by insertional fusion Richard John Ward, Lucas Ribeiro, Gilvan Furtado and Marcos Lourenzoni 11. β-Etherases in lignin valorization Anett Schallmey, Margarita Seeger and Julian Pagel 12. Strategies for lignocellulosic hydroxycinnamic acid release and biotransformation into high-value coniferyl alcohol using feruloyl esterase and double reduction whole cell biocatalyst Fabio Squina and Robson Tramontina 13. Feruloyl-CoA synthetases and feruloyl-CoA hydratase/lyases: expression, biochemical characterization, and generation of vanillin from ferulic acid and lignocellulosic hydrolysates Fabio Squina and Victoria Sodré 14. Glycolate oxidase from Rhodococcus jostii RHA1: an accessory enzyme for bacterial lignin degradation Awatif H. Alruwaili and Timothy David Bugg 15. Characterization of bacterial transporters involved in lignin-derived aromatic compounds Eiji Masai, Masaya Fujita and Naofumi Kamimura 16. Expression and activity of aryl-alcohol oxidase Juan Carro Aramburu 17. Ligninolytic peroxidases: a guide for their heterologous expression in E. coli and protocols to evaluate their activity on lignin María Isabel Sánchez-Ruiz, Iván Ayuso-Fernández, Dolores Linde and Francisco Javier Ruiz-Dueñas 18. Assessing Iron Reductase Activity: Methodologies for Fungal Degradation of Lignocellulose Irnia Nurika