Ion and Molecule Transport in Lysosomes

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Bruno Gasnier (Paris Descartes University, France) Michael X. Zhu (University of Texas, Health Science Center at Houston, Houston, Texas, USA)

Ion and Molecule Transport in Lysosomes

Bruno Gasnier (Paris Descartes University, France), Michael X. Zhu (University of Texas, Health Science Center at Houston, Houston, Texas, USA)

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English

CRC Press
01 February 2022

Medicine: general issues; Anatomy; Molecular biology; Cellular biology (cytology)

Series: Methods in Signal Transduction Series

Lysosomes are key subcellular organelles that regulate the cell function. Many of the essential activities of the cell are dependent on lysosomes. Dysfunction is linked to multiple diseases - storage disorders, neurodegeneration, immunological diseases and cancer. This book discusses concepts and methods used to study lysosome ion and small molecule transport. The contents will not only attract accomplished investigators in need of a broad review and synthesis of this important subject but will also appeal to young investigators and trainees needing to acquire comprehensive knowledge and technical skills working with lysosomal ion channels and small molecule transporters.

Key selling features:

Summarizes the endocellular role that lysosomes play with respect to cellular waste disposal Reviews essential cellular functions of lysosomes Explores how lysosome dysfunction is the cause of many metabolic disorders Examines how lysomes are involved in storage diseases Describes various technologies and methods used in lysosome research

Edited by: Bruno Gasnier (Paris Descartes University France), Michael X. Zhu (University of Texas, Health Science Center at Houston, Houston, Texas, USA)
Imprint: CRC Press
Country of Publication: United Kingdom
Dimensions: Height: 234mm, Width: 156mm,
Weight: 421g
ISBN: 9781032241159
ISBN 10: 1032241152
Series: Methods in Signal Transduction Series
Pages: 252
Publication Date: 01 February 2022
Audience: College/higher education , General/trade , Primary , ELT Advanced
Format: Paperback
Publisher's Status: Active

Preface. Editors. Contributors. Endosomal and Lysosomal Electrophysiology. Chloride Transport across the Lysosomal Membrane. Endolysosomal Patch Clamping: Approaches to Measure Vesicular Ion Channel Activities. TRPML Subfamily of Endolysosomal Channels: Concepts and Methods. Investigating the Role of Two-Pore Channel 2. (TPC2) in Zebrafish Neuromuscular Development. Functional Study of Lysosomal Nutrient Transporters. Lysosomal Vitamin B12. Trafficking. Detection of Lysosomal Membrane Permeabilization. Rapid Isolation of Lysosomes from Cultured Cells Using a Twin Strep Tag. A Transcriptomic Analysis and shRNA Screen for Intracellular Ion Channels and Transporters Regulating Pigmentation. Index

Bruno Gasnier is CNRS Research Director, Group Leader at CNRS/Paris Descartes. Michael Xi Zhu is currently with the Department of Integrative Biology and Pharmacology at the University of Texas Health Science Center at Houston.