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Introduction to Fluorescence

David M. Jameson

$179

Hardback

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English
CRC Press Inc
22 January 2014
A concise introduction to fundamental principles, this book presents the most important aspects of fluorescence spectroscopy, particularly as they are applied to biology, biochemistry, and biophysics. The author focuses on the essential aspects of fluorescence to encourage readers to design experiments, begin their own fluorescence measurements, and access the literature with greater comprehension. After a general definition of the field and an overview of the nature of light and absorption by biomolecules, the text covers instrumentation, emission spectra, excitation spectra, polarization, anisotropy, quenching, time-resolved methods, and FRET.

By:  
Imprint:   CRC Press Inc
Country of Publication:   United States
Dimensions:   Height: 234mm,  Width: 156mm,  Spine: 18mm
Weight:   750g
ISBN:   9781439806043
ISBN 10:   1439806047
Pages:   314
Publication Date:  
Audience:   College/higher education ,  General/trade ,  Primary ,  ELT Advanced
Replaced By:   9781032261713
Format:   Hardback
Publisher's Status:   Active
Introduction. Absorption of Light. Instrumentation. Emission and Excitation Spectra. Polarization and Anisotropy. Time-Resolved Fluorescence. Quantum Yields and Quenching. Förster Resonance Energy Transfer. Brief Overview of Fluorescence Microscopy. Fluorophores. Intrinsic Protein Fluorescence. Appendix. Index.

University of Hawaii, Manoa, USA

Reviews for Introduction to Fluorescence

An essential contribution to educating scientists in the principles of fluorescence. It will also be an important addition to the libraries of practitioners applying the principles of molecular fluorescence. -Ken Jacobson, Kenan Distinguished Professor of Cell Biology and Physiology, University of North Carolina at Chapel Hill An exquisite compendium of fluorescence and its applications in biochemistry enriched by a very exciting historical perspective. This book will become a standard text for graduate students and other scientists. -Drs. Zygmunt (Karol) Gryczynski and Ignacy Gryczynski, University of North Texas Health Science Center ... truly a masterwork, combining clarity, precision, and good humor. The reader, novice or expert, will be pleased with the text and will not stop reading. It is a formidable account of the fluorescence field, which has impacted the life sciences so considerably in the last 60 years. -Jerson L. Silva, M.D., Ph.D., Professor and Director, National Institute of Science and Technology for Structural Biology and Bioimaging, Universidade Federal do Rio de Janeiro Dr. Jameson's book fills a gap in the literature available for the student approaching the applications of fluorescence techniques as well as for the researcher practicing them daily in the laboratory setting. The subject is presented by the author in a clear didactic manner; the anecdotes and historical references make the book truly a delight to read. -Dr. Beniamino Barbieri, President, ISS Inc. An excellent textbook! This comprehensive overview written at an introductory level covers fundamental aspects, principles of instrumentation, and practical applications of biological fluorescence, while providing many valuable tips. In essence, this book reflects the excellent didactic skills and vast experience of the author in research and teaching fluorescence. Highly recommendable for undergraduate teaching. Also excellent material for active researchers who are learning to apply fluorescence methods. -Prof. Luis Bagatolli, Center for Biomembrane Physics, Department of Biochemistry and Molecular Biology, University of Southern Denmark I am very impressed by the author's lucid treatment. This book is a 'must read' for students and researchers. -Prof. Amitabha Chattopadhyay, Ph.D., FRSC, J.C. Bose Fellow, Outstanding Scientist, Centre for Cellular & Molecular Biology, Hyderabad This introductory book will be valuable to the scientific community as well as to the amateur microscopist/spectroscopist. -Prof. Michelle Digman, Department of Biomedical Engineering and Laboratory for Fluorescence Dynamics, University of California, Irvine


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