Intracellular Thermometry with Fluorescent Molecular Thermometers

Seiichi Uchiyama (University of Tokyo, Japan)

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English

Blackwell Verlag GmbH
24 April 2024

Thermodynamics & heat; Molecular biology; Cellular biology (cytology)

Intracellular Thermometry with Fluorescent Molecular Thermometers Understand a vital new bioanalytical technique with this comprehensive introduction to measuring temperature on the cellular scale

Most organisms have highly controlled body temperatures, fluctuations in which are therefore sensitive indicators of changes in body function. In recent years, the development of fluorescent molecular thermometers and related intracellular temperature probes has enabled researchers to track these fluctuations at the cellular rather than the organismic level, opening up a whole new field of study in cell and molecular biology.

Intracellular Thermometry with Fluorescent Molecular Thermometers provides bioanalytical researchers with an introduction to these technologies and their current and future applications. Starting off with a discussion of temperature as a key factor in biological regulation, it provides an authoritative overview of available fluorescent temperature probes, their characteristics and potential applications.

Intracellular Thermometry with Fluorescent Molecular Thermometers readers will also find:

Step by step instructions for constructing an intracellular thermometry experiment and validating results Comprehensive discussion of existing applications A vision for the future development of thermal biology as an independent discipline

Authored by a pioneer in the field of intracellular thermometry, Intracellular Thermometry with Fluorescent Molecular Thermometers is ideal for researchers in analytical chemistry, cell biology, molecular biology, biophysics, or any related subjects.

By: Seiichi Uchiyama (University of Tokyo Japan)
Imprint: Blackwell Verlag GmbH
Country of Publication: Germany
Dimensions: Height: 244mm, Width: 170mm, Spine: 24mm
Weight: 794g
ISBN: 9783527350322
ISBN 10: 3527350322
Pages: 352
Publication Date: 24 April 2024
Audience: Professional and scholarly , Undergraduate
Format: Hardback
Publisher's Status: Active

"TEMPERATURE IN BIOLOGICAL SPECIES Definition of temperature Body temperature Effects of environmental temperature on biological functions FLUORESCENT PROBES FOR MEASURING TEMPERATURE Physical chemistry of fluorescence Fluorescent molecular thermometers - small organic molecules - organometallic complexes - inorganic materials - macromolecules (including fluorescent proteins) INTRACELLULAR THERMOMETRY Endogenous thermogenesis Effects of external stimuli on intracellular temperature Comparable non-fluorescent technique in intracellular thermometry Validation of intracellular thermometry Relationship between intracellular temperature and biological events BEYOND INTRACELLULAR THERMOMETRY ""Thermal biology"" In vivo internal thermometry of living species Future perspectives"

Seiichi Uchiyama, PhD, is an Assistant Professor at the University of Tokyo, Japan. He received his academic degrees in Pharmacy from the University of Tokyo. Following this, he was a postdoctoral researcher at Nara Women's University and Queen's University of Belfast. His research interests include analytical and photophysical chemistry and the development of fluorescent sensors based on novel functional mechanisms.