Integrated Methods in Protein Biochemistry

Part B

Arun K. Shukla (Indian Institute of Technology, Kanpur, India)

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English

Academic Press Inc
18 January 2023

Biochemistry; Molecular biology

Series: Methods in Enzymology

Integrated Methods in Protein Biochemistry: Part B, Volume 678 in the Methods in Enzymology series, highlights new advances in the field, with this new volume presenting interesting chapters on a variety of topics, including Precise modification of native proteins, purification, and analysis of bioconjugates, NanoBiT-based methods to monitor the activation and modulation of RTKs, The interplay of G-protein ß? subunit and PLC-ß enzyme in PIP2 hydrolysis and downstream signaling, Structure and function of bacterial secretion system, Tools and protocols for probing protein sumoylation, Spectroscopic analysis of cysteine dioxygenase: a mammalian thiol-dioxygenase, DeGlyPHER: MS-based analysis of viral spike N-glycoforms, and more.

Additional sections cover Covalent protein painting: MS-based protein footprinting, Characterization of GPCR signaling complexes using negative-staining electron microscopy, Probing protein misfolding and dissociation with free electron laser, Optimized protocol for the characterization of Cas12a activities, Proximity proteomics for the identification and characterization of extracellular vesicles, Structural and Functional characterization of lytic polysaccharide monooxygenases, and much more.

Volume editor: Arun K. Shukla (Indian Institute of Technology Kanpur India)
Imprint: Academic Press Inc
Country of Publication: United Kingdom
Dimensions: Height: 229mm, Width: 152mm,
Weight: 790g
ISBN: 9780323992640
ISBN 10: 0323992641
Series: Methods in Enzymology
Pages: 420
Publication Date: 18 January 2023
Audience: Professional and scholarly , Undergraduate
Format: Hardback
Publisher's Status: Active

Dr. Arun K. Shukla is a world leader in the field of GPCR biology and he is currently a Professor in the Department of Biological Sciences and Bioengineering at the Institute of Technology, Kanpur in India. Dr. Shukla’s research program is focused on understanding the structure, function and regulation of G protein-coupled receptors with a long-term of designing novel therapeutics with minimized side-effects.