In Vivo Cellular Imaging Using Fluorescent Proteins

Methods and Protocols

Robert Hoffman

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English

Humana Press Inc.
11 June 2012

Cellular physiology; Medical imaging; Cellular biology (cytology)

Series: Methods in Molecular Biology

The discovery and genetic engineering of fluorescent proteins has revolutionized cell biology. What was previously invisible in the cell often can be made visible with the use of fluorescent proteins. In Vivo Cellular Imaging Using Fluorescent Proteins: Methods and Protocols presents state-of-the-art research that has contributed to the fluorescent protein revolution to visualize biological processes in the live animal. This volume covers an array of topics from the employment of the chick CAM model using fluorescent proteins and other fluorescent probes, to intravital fluorescent imaging, as well as 3-dimensional imaging, and design instructions on how to create new and improved far-red and infrared fluorescent proteins, to name a few. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, In Vivo Cellular Imaging Using Fluorescent Proteins: Methods and Protocols is the first volume in the new field of in vivo cell biology and it serves both professionals and novices with its well-honed methodologies.

Edited by: Robert Hoffman
Imprint: Humana Press Inc.
Country of Publication: United States
Edition: 2012 ed.
Volume: 872
Dimensions: Height: 254mm, Width: 178mm, Spine: 23mm
Weight: 736g
ISBN: 9781617797965
ISBN 10: 1617797960
Series: Methods in Molecular Biology
Pages: 269
Publication Date: 11 June 2012
Audience: Professional and scholarly , Undergraduate
Format: Hardback
Publisher's Status: Active

Reviews for In Vivo Cellular Imaging Using Fluorescent Proteins: Methods and Protocols

From the reviews: “Robert M. Hoffman introduces In vivo Cellular Imaging Using Fluorescent proteins, the eighteen chapters book dedicated to the description of how fluorescence proteins have changed the way to analyze cellular processes in vivo. … This book is very well structured with several colored figures … . this is a useful book not only for those who study the biology of different cancer cells, but also for those who are interested in following the in vivo dynamic of cellular processes.” (Manuela Monti, European Journal of Histochemistry, Vol. 56, 2012)