Immunofluorescence in Clinical Immunology

A Primer and Atlas

Wulf B. Storch, R.A. Klein

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English

Birkhauser Verlag AG
01 April 2000

Immunology; Biochemical immunology; Molecular biology; Cellular biology (cytology)

Immunofluorescence, a suitable laboratory method for the

microscopic demonstration of antigens and antibodies in biological

materials, useable, for example, to provide evidence for the

pathogenesis of disease in histological or cytological preparations and

for tumour cell differentiation. For this reason immunofluorescence has

a decisive role as the method of choice for the diagnosis of auto-immune

diseases. This primer on immunofluorescence techniques, which first

appeared in 1979, is a richly illustrated handbook suitable for everyday

practical work in the laboratory, useable as both an introduction to the

subject as well as an atlas. In hardly any other area of medicine are

there so many new findings to report. The second edition of this book is

concerned not only with the detection methods which now form an

essential and established part of diagnostic techniques, but also with

the most recent research results such as the discovery of antibodies

against Auerbach's plexus and against podocytes...

By: Wulf B. Storch
Translated by: R.A. Klein
Imprint: Birkhauser Verlag AG
Country of Publication: Switzerland
Edition: 3rd Revised edition
Dimensions: Height: 254mm, Width: 178mm, Spine: 20mm
Weight: 860g
ISBN: 9783764361822
ISBN 10: 3764361824
Pages: 231
Publication Date: 01 April 2000
Audience: College/higher education , Professional and scholarly , Further / Higher Education , Undergraduate
Format: Hardback
Publisher's Status: Active

1 Introduction to the basic principles and techniques of immunofluor-escence.- 2 Production of antibodies and labelling with fluorochromes.- 3 Characterisation and quality control of antisera and conjugates.- 4 Actual optical immunofluorescence techniques.- 5 Instrumental technique and the documentation of findings.- 6 General advice.- 7 Applications of immunofluorescence.