High-Performance Liquid Chromatography of Peptides and Proteins: Separation, Analysis, and Conformation...

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Colin T. Mant Robert S. Hodges

High-Performance Liquid Chromatography of Peptides and Proteins

Separation, Analysis, and Conformation

Colin T. Mant, Robert S. Hodges

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English

CRC Press
17 October 2019

Chromatography; Life sciences: general issues

This book consists of a series of 82 precise, easy-to-read articles by internationally renowned scientists

and emphasizes the practical approach to HPLC with minimal theory, although the underlying principles for peptide and protein separations are clearly expressed. All of the major modes of microbore, ultrafast and analytical HPLC are discussed, including size-exclusion, ion-exchange, reversed-phase, hydrophobic interaction, and affinity and immunoaffinity chromatography. A section on preparative HPLC, including displacement techniques, is also presented. Problem-solving approaches to the separation of various classes of biologically active peptides and proteins are thoroughly explored, while the importance of peptide standards for monitoring column performance and for optimizing separation conditions is emphasized. Several articles focus on the choice of the correct detection method (electrochemical, UV, fluorescence), as well as the need for a proper knowledge of approaches to column and instrument maintenance and trouble-shooting. A section on predictive approaches deals with both computer simulation of peptide separations and peptide structure. The book also includes complementary techniques to HPLC, as well as other useful applications of HPLC. It enables both novice and experienced chromatographers to realize the full potential of this extremely powerful technique, in the process making an important contribution to scientific literature.

Edited by: Colin T. Mant, Robert S. Hodges
Imprint: CRC Press
Country of Publication: United Kingdom
Dimensions: Height: 254mm, Width: 178mm,
Weight: 453g
ISBN: 9780367402969
ISBN 10: 0367402963
Pages: 960
Publication Date: 17 October 2019
Audience: Professional and scholarly , Undergraduate
Format: Paperback
Publisher's Status: Active

Colin T. Mant, Robert S. Hodges