Heterologous Expression of Membrane Proteins

Methods and Protocols

Isabelle Mus-Veteau

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English

Springer-Verlag New York Inc.
01 July 2022

Biochemistry; Proteins

Series: Methods in Molecular Biology

Summary
Details

This detailed volume explores protocols for the production of membrane proteins in a panel of heterologous organisms for structural studies. Beginning with techniques using E. coli as a host for the overproduction and purification of membrane proteins, the book continues with chapters covering mammalian membrane protein production in yeast, insect cells, mammalian cells, as well as using virus like particles and acellular systems. Additionally, new detergents and alternatives to detergents allowing membrane protein purification for structural analyses are described. The book closes with a chapter exploring the use of microscale thermophoresis (MST) to evaluate the binding activity of heterologously expressed proteins directly in crude membrane extracts. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratoryprotocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Heterologous Expression of Membrane Proteins: Methods and Protocols, Third Edition serves as an ideal guide for scientists aiming to produce and purify functional recombinant membrane proteins for structural studies.

Edited by: Isabelle Mus-Veteau
Imprint: Springer-Verlag New York Inc.
Country of Publication: United States
Edition: 3rd ed. 2022
Volume: 2507
Dimensions: Height: 254mm, Width: 178mm,
Weight: 1.107kg
ISBN: 9781071623671
ISBN 10: 1071623672
Series: Methods in Molecular Biology
Pages: 467
Publication Date: 01 July 2022
Audience: Professional and scholarly , Undergraduate
Format: Hardback
Publisher's Status: Active