Fluorescence Sensors and Biosensors

Richard B. Thompson, Richard B. Thompson, Douglas A. Christensen (University of Utah, Salt Lake City, USA) , Weihong Tan (University of Florida, Gainesville, Florida, USA)

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English

CRC Press Inc
16 December 2005

Medical equipment & techniques; Medical research; Analytical chemistry; Biology, life sciences; Biosensors; Pharmaceutical technology

Fluorescence-based sensing is a significant technique used in prominent fields such as fluorescence-activated cell sorting, DNA sequencing, high-throughput screening, and clinical diagnostics. Fluorescence Sensors and Biosensors emphasizes the most recent developments and emerging technologies with the broadest impacts.

The text begins with the development of aptamers (oligoribonucleotides) and biorecognition techniques based on periplasmic binding proteins. The following chapters review the molecular beacon approach for DNA recognition, describe resonance energy transfer (FRET) in sensing, and present the use of carbonic anhydrase recognition platform for metal ion determination and imaging. The book explores the advantages of fluorophores, fluorescent labels, sensor and assay construction, metal-enhanced fluorescence, phosphorescent labels, and lab-on-a-chip applications. It also describes new anion-selective fluorescent probes used as analytes in clinical determinations.

The final chapters highlight the application of fluorescence sensing technology to several practical problems, such as the development of planar waveguide biosensors for clinical diagnostics and the adaptation of fluorescence-based sensing approaches for biochemical production by fermentation. The book also discusses the measurement of analytes, such as free zinc ions, at ultratrace levels in biological specimens. Written by internationally renowned authors in their fields, Fluorescence Sensors and Biosensors provides an up-to-date account of fluorescence-based sensors focused on practical applications in biotechnology, analytical chemistry, and biomedicine.

Contributions by: Douglas A. Christensen (University of Utah Salt Lake City USA), Weihong Tan (University of Florida, Gainesville, Florida, USA), Zeev Rosenzweig (University of New Orleans, New Orleans, Louisiana, USA)
Edited by: Richard B. Thompson, Richard B. Thompson
Imprint: CRC Press Inc
Country of Publication: United States
Dimensions: Height: 234mm, Width: 156mm, Spine: 27mm
Weight: 725g
ISBN: 9780824727376
ISBN 10: 0824727371
Pages: 420
Publication Date: 16 December 2005
Audience: Professional and scholarly , Professional and scholarly , Professional & Vocational , Undergraduate , Further / Higher Education
Format: Hardback
Publisher's Status: Active

Introduction; Prospects for the De Novo Design of Nucleic Acid Biosensors; Biosensors Based on Periplasmic Binding Proteins; Molecular Beacon DNA Probes Based on Fluorescence Biosensing; Fluorescence Resonance Energy Transfer-Based Sensors for Bioanalysis; Carbonic Anhydrase-Based Biosensing of Metal Ions: Issues and Future Prospects; Metal-Enhanced Fluorescence Sensing; Subpicomolar Assays of Antibodies and DNA Using Phosphorescence Labels; Development of Fluorescent Dipyrrolylquinoxaline-Based Anion Sensors;Lab-on-a-Chip and Fluorescence Sensing on the Microscale; The Array Biosensors; Planar Waveguide Biosensors for Point-of-Care Clinical and Molecular Diagnostics; Fluorescence-Based Sensors for Bioprocess Monitoring; Practical Aspects of Fluorescence Analysis of Free Zinc Ion in Biological Systems: pZn for the Biologist;

Richard B.Thompson

Reviews for Fluorescence Sensors and Biosensors

“… emphasizes the most recent developments and emerging technologies with the broadest impacts in prominent fields such as fluorescence-activated cell sorting, DNA sequencing, high throughput screening, and clinical diagnostics. Roughly grouped under three headings, namely new recognition or transduction approaches, other new technology, and selected applications, …The book also discusses the measurement of analytes, such as free zinc ions, at ultratrace levels in biological specimens. Written by internationally renowned authors in their fields, … provides an up-to-date account of fluorescence-based sensors focused on practical applications in biotechnology, analytical chemistry, and biomedicine.” — J Albaigés, Editor in Chief, Department of Environment Chemistry CID-CSIC, Barcelona, Spain, in International Journal Of Environmental Analytical Chemistry, Vol. 86, 2006 ""Written by internationally renowned authors in their fields, this book provides an up-to-date account of fluorescence-based sensors focused on practical applications in biotechnology, analytical chemistry, and biomedicine."" – In Anticancer Research, July/ August 2008, Vol. 28, No. 4A