Enzyme Engineering

Methods and Protocols

Francesca Magnani, Chiara Marabelli, Francesca Paradisi

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English

Springer-Verlag New York Inc.
25 November 2022

Chemistry; Enzymology; Biotechnology

Series: Methods in Molecular Biology

Summary
Details

This book provides comprehensive methods and protocols about enzyme design. The chapters are grouped by main topic, starting with methodologies describing library preparation and screening, state of the art techniques in directed evolution and rational design, followed by examples of immobilization of enzymes on sustainable polymers, as well as biocatalytic conversions mediated by homogenous enzymatic preparations or whole cells. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and timely, Enzyme Engineering: Methods and Protocols is an ideal guide for both the novice and the veteran researcher interested in biocatalysis. Chapter 13 is available open access under a CC BY 4.0 license.

Edited by: Francesca Magnani, Chiara Marabelli, Francesca Paradisi
Imprint: Springer-Verlag New York Inc.
Country of Publication: United States
Edition: 2022 ed.
Volume: 2397
Dimensions: Height: 254mm, Width: 178mm,
Weight: 706g
ISBN: 9781071618288
ISBN 10: 1071618288
Series: Methods in Molecular Biology
Pages: 360
Publication Date: 25 November 2022
Audience: Professional and scholarly , Undergraduate
Format: Paperback
Publisher's Status: Active