Methods in Enzymology, Volume 644, the latest release in this ongoing serial, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Chapters in this new release include Site-directed recombination (SDR) in vivo: a fast and reliable tool to unveil beneficial epistasis, Creation and application of amine oxidase with expanded substrate specificities from porcine kidney D-amino acid oxidase, Methods to assess correlation networks for engineering transketolase, Exploration of Enzyme Diversity by Integrating Bioinformatics with Microfluidics, Engineering lytic polysaccharide monooxygenases (LPMOs), Emulsion-based directed evolution of enzymes in yeast, and much more.
1. Engineering lytic polysaccharide monooxygenases (LPMOs) Zarah Forsberg, Anton A. Stepnov, Guro Kruge Nærdal, Geir Klinkenberg and Vincent G. H. Eijsink 2. Strategies for the expression and characterization of artificial myoglobin-based carbene transferases Daniela M. Carminati, Eric J. Moore and Rudi Fasan 3. Methods for the detection and analysis of dioxygenase catalyzed dihydroxylation in mutant derived libraries Julian L. Wissner, Wendy Escobedo-Hinojosa, Peter M. Heinemann, Andreas Hunold and Bernhard Hauer 4. Enhancing promiscuous chemistries of a Schiff-base forming enzyme by divergent evolution Duncan S. Macdonald and Donald Hilvert 5. Glutamine-walking: Creating reactive substrates for transglutaminase-mediated protein labeling Lukas Deweid, Adem H. Parisien, Kiana Lafontaine, Léa N.C. Rochet, Harald Kolmar and Joelle N. Pelletier 6. Engineering aldolases for asymmetric synthesis Mikael Widersten 7. Ultra-high throughput screening for novel protease specificities Eirini Rousounelou, Steven Schmitt, Luzius Pestalozzi, Martin Held, Tania M. Roberts and Sven Panke 8. Directed evolution of transcriptional switches using dual-selector systems Yuki Kimura and Daisuke Umeno 9. Methods for competitive enrichment and evaluation of superior DNA ligases Janine K. Sharma, Luke J. Stevenson, Katherine J. Robins, Adele K. Williamson, Wayne M. Patrick and David F. Ackerley 10. Directed evolution of custom polymerases using droplet microfluidics Derek Vallejo, Ali Nikoomanzar and John C. Chaput 11. Optically guided mass spectrometry to screen microbial colonies for directed enzyme evolution Pu Xue, Tong Si and Huimin Zhao 12. Split enzymes: Design principles and strategy Shion A. Lim and James A. Wells
Dan Tawfik is Principal Investigator at The Department of Biomolecular Sciences, Weizmann Institute of Science, Israel.
