DNA Damage Detection

Modified Alkaline Comet Assay to Detect Oxidative DNA Damage.- Radiation-Induced DNA Strand Break Detection by Gel Electrophoresis.- Quantitative Measurement of 8-Hydroxy-2′-Deoxyguanosine in Serum/Plasma and Urine Using an Enzyme-Linked Immunosorbent Assay.- Radiation-Induced Gamma-H2AX Foci Staining and Analysis.- Non-Radiolabeled Radioresistant DNA Synthesis and S-Phase Checkpoint Analysis.- Constant Field Gel Electrophoresis Analysis for DNA Double-Strand Break and Its Repair.- Detection of Chromatid Break and Micronucleus Formation Induced by Low- and High-LET Irradiation.- Measurement of DNA Polymerase and Microhomology-Mediated End-Joining Activities by Gel Electrophoresis and smFRET Techniques.- UV-Induced DNA Damage Detection by ELISA Analysis.- G2 Premature Chromosome Condensation (G2-PCC) by Calyculin A to Analyze DNA Double-Strand Breaks and Their Repair.- Mitotic Shake-Off and Cell Cycle Synchronization.- DCFDA Assay for Oxidative Stress Measurement in Fluorometer.- HPRT Mutation Assay for Chinese Hamster Ovary Cells.- Detection of De Novo Mutations by Sequencing Reduced Representation Libraries.- Chemical-Induced DNA Damage Identification by High-Throughput Detection of DNA Double-Strand Break.- Assessing DNA Damage Through the Cell Cycle Using Flow Cytometry.- A Mass Spectrometry-Based Proteomics Workflow for Concurrent Profiling of Protein Thiol Oxidation and Phosphorylation.- Analyzing Homologous Recombination Using Antibiotic Marker Substrates in Mammalian Cells.