Directed Evolution

Methods and Protocols

Andrew Currin, Neil Swainston

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English

Springer-Verlag New York Inc.
23 June 2023

Medical genetics; Biotechnology

Series: Methods in Molecular Biology

Summary
Details

This volume explores the latest techniques used by researchers to study directed evolution (DE) at each stage of the Design-Build-Test-Learn cycle. Chapters in this book cover topics such as designing overlap extension PCR primers for protein mutagenesis; antha-guided automation of Darwin assembly for the construction of bespoke gene libraries; rapid cloning of random mutagenesis libraries using PTO-Quickstep; and DE of glycosyltransferases by a single-cell screening method. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Directed Evolution: Methods and Protocols is a valuable resource for scientists and researchers who are interested in learning more about this field and incorporating these studies into new experimental workflows.

Edited by: Andrew Currin, Neil Swainston
Imprint: Springer-Verlag New York Inc.
Country of Publication: United States
Edition: 2022 ed.
Volume: 2461
Dimensions: Height: 254mm, Width: 178mm,
Weight: 559g
ISBN: 9781071621547
ISBN 10: 1071621548
Series: Methods in Molecular Biology
Pages: 278
Publication Date: 23 June 2023
Audience: Professional and scholarly , Undergraduate
Format: Paperback
Publisher's Status: Active