This volume discusses the latest advancements and techniques used to study Dictyostelium discoideum. The chapters in this book cover topics such as cytokinesis, membrane function and damage, and nutrient/bacterial processing; autophagy activation and function; protein localization, interaction, and activation; cell migration and contact guidance for quantification of cytoskeletal dynamics; and global mutational screening, transcriptome analyses, and proteomics that apply to all stages of the Dictyostelium life cycle and that may generalize to human disease models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and comprehensive, Dictyostelium discoideum: Methods and Protocols is a valuable resource for all researchers who are interested in learning more about this important and developing field.
Edited by:
Alan R. Kimmel Imprint: Humana Country of Publication: United States Edition: 2024 ed. Volume: 2814 Dimensions:
Height: 254mm,
Width: 178mm,
ISBN:9781071638934 ISBN 10: 1071638939 Series:Methods in Molecular Biology Pages: 258 Publication Date:03 July 2024 Audience:
Professional and scholarly
,
Undergraduate
Format:Hardback Publisher's Status: Active